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Molecular cloning and characterization of starch-branching enzyme II from potato

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Abstract

Full-length cDNA for starch branching enzyme (SBE) II of potato was isolated and sequenced. In potato, similary to most other investigated plants, the SBE-II isoform differs from SBE-I by having an acidic amino-terminal extension and a shorter carboxyterminus. Two forms of SBE-II, migrating as 98 and 95 kDa proteins in 6% SDS-polyacrylamide gels, were associated to tuber starch. The latter form was 16 amino acids shorter in the amino terminus. Transcript of SBE-II was present in leaf tissue, whereas significant expression was not seen in tubers. On the other hand, a significant amount of SBE-I transcript was detected in tuber tissue but not in leaves.

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Authors and Affiliations

  1. Uppsala Genetic Center, Dept. of Cell Research, Swedish University of Agricultural Sciences, Uppsala, Sweden

    Clas-Tomas Larsson, Jamshid Khoshnoodi, Bo Ek & Håkan Larsson

  2. Dept. of Medical and Physiological Chemistry, Uppsala University, Uppsala, Sweden

    Lars Rask

Authors
  1. Clas-Tomas Larsson
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  2. Jamshid Khoshnoodi
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  3. Bo Ek
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  4. Lars Rask
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  5. Håkan Larsson
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Larsson, CT., Khoshnoodi, J., Ek, B. et al. Molecular cloning and characterization of starch-branching enzyme II from potato. Plant Mol Biol 37, 505–511 (1998). https://doi.org/10.1023/A:1005908305456

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