Abstract
We have examined in vitro rooting of apple ‘Jork 9‘ shoots exposed for three weeks to each of the three auxins commonly used for ex vitro rooting: indole-3-acetic acid (IAA), indole-3-butyric acid (IBA) and α-naphthaleneacetic acid (NAA). During the initial five days of the rooting treatment, the cultures were incubated in darkness. In this period, the root initials are formed. Then, the cultures were moved to the light. NAA resulted in a low (ca. 8 roots), and IAA or IBA in a high (ca. 15 roots) maximal root number. The maximal root number was reached at a wide range of IAA concentrations (10-100 μM) but at only one concentration of IBA (10 μM) or NAA (3 μM). With NAA and IBA, growth of roots and shoots was much more inhibited than with IAA. For these reasons, IAA is the preferable auxin for in vitro rooting of apple ‘Jork 9’ shoots.
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Boxus P (1974) —La culture deméristèmes de Prunus pour l'obtention de plants sains. Acta Hortic. 44: 43–46
De Klerk GJ, Ter Brugge J, Smulders R & Benschop M (1990) Basic peroxidases and rooting in microcuttings of Malus. Acta Hortic. 280: 29–36
De Klerk GJ & Caillat E (1994) Rooting responses of stem-disks excised from the same ‘M9 Jork’ microcutting. Adv. Hort. Sci. 8: 15–18
De Klerk GJ, Keppel M, Ter Brugge J & Meekes H (1995) Timing of the phases in adventitious root formation in apple microcuttings. J. Exp. Bot. 46: 965–972
Dunlap JR, Kresovich S & McGee RE (1986) The effect of salt concentration on auxin stability in culture media. Plant Physiol. 81: 934–936
Epstein E & Ludwig-Müller J (1993) Indole-3-butyric acid in plants: occurrence, synthesis, metabolism and transport. Physiol. Plant. 88: 382–389
George EF (1996) Plant Propagation by Tissue Culture. Part 2. In Practice. Exegetics Ltd., Eddington
Hartmann HT, Kester DE & Davies FT (1990) Plant Propagation: Principles and Practices, Prentice Hall, Englwood Cliffs, NJ
Jasik J & De Klerk GJ (1997) Anatomical and ultrastructural examination of adventitious root formation in stem slices of apple. Biol. Plant. 39, 79–90
Mohammed GH & Vidaver WE (1990) The influence of acclimatization treatment and plantlet morphology on early greenhouse performance of tissue-cultured Douglas fir [Pseudotsuga menziesii (Mirb.) Franco]. Plant Cell Tiss. Org. Cult. 21: 111–117
Nissen SJ & Sutter EG (1990) Stability of IAA and IBA in nutrient medium of several tissue culture procedures. HortScience 25: 800–802
Peeters AJM, Gerads W, Barendse GWM & Wullems GJ (1991) In vitro flower bud formation in tobacco: interaction of hormones. Plant Physiol. 97: 402–408
Smulders MJM, Van De Ven ETWM, Croes AF & Wullems GJ (1990) Metabolism of 1-naphthaleneacetic acid in explants of tobacco: evidence for release of free hormone from conjugates. J. Plant Growth Regul. 9: 27–34
Van Der Krieken W M, Breteler H, Visser MHM & Mavridou D (1993) The role of conversion of IBA into IAA on root regeneration in apple: introduction of a test system. Plant Cell Rep. 12: 203–206
Van Telgen HJ, Van Mil A & Kunneman B (1992) Effect of propagation and rooting conditions on acclimatization of micropropagated plants. Acta Bot. Neerl. 41: 453–460
Wisniewski LA, Frampton LJ & McKeand SE (1986) Early shoot and root quality effects on nursery and field development of tissuecultured loblolly pine. HortScience 21: 1185–1186
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De Klerk, GJ., Brugge, J.T. & Marinova, S. Effectiveness of indoleacetic acid, indolebutyric acid and naphthaleneacetic acid during adventitious root formation in vitro in Malus ‘Jork 9’. Plant Cell, Tissue and Organ Culture 49, 39–44 (1997). https://doi.org/10.1023/A:1005850222973
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DOI: https://doi.org/10.1023/A:1005850222973