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Promoter sequences from two different Brassica napus tapetal oleosin-like genes direct tapetal expression of β-glucuronidase in transgenic Brassica plants

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Abstract

To investigate the sequences responsible for the regulated expression of tapetal-specific oleosin-like genes, ca. 2 kb of the 5′-upstream regions from two divergent genes, OlnB;4 and OlnB;13, were isolated, sequenced and fused to the reporter gene β-glucuronidase for study in transgenic Brassica napus plants. Although the proteins encoded by these two genes are highly divergent, except for the conserved oleosin-like domain, the first 250 bp of their 5′-upstream regions was 86% identical, including a region of 150 bp upstream from the TATA box. Analysis of 42 independent transformants by histochemical and fluorometric methods showed that both promoters directed tapetal-specific expression that peaked at the 4 mm flower bud stage.

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Author notes

  1. Stamatis Rigas & Polydefkis Hatzopoulos

    Present address: Molecular Biology Department, Agricultural University of Athens, Greece

Authors and Affiliations

  1. Plant Biotechnology Institute, National Research Council, 110 Gymnasium Rd, Saskatoon, Saskatchewan, S7N 0W9, Canada

    Hai Ping Hong, Raju S.S. Datla & Wilf Keller

  2. Department of Brassica and Oilseeds Research, John Innes Centre, Colney Lane, Norwich, NR4 7UH, UK

    Joanne H.E. Ross & Denis J. Murphy

  3. Eastern Cereal and Oilseed Research Centre, Central Experimental Farm, Ottawa, Ontario, K1A 0C6, Canada

    Jean L. Gerster & Laurian S. Robert

  4. Department of Crop Science and Plant Ecology, University of Saskatchewan, Saskatoon, Saskatchewan, S7N 5A8, Canada

    Graham Scoles

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  1. Hai Ping Hong
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  4. Stamatis Rigas
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  5. Raju S.S. Datla
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  6. Polydefkis Hatzopoulos
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  7. Graham Scoles
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  8. Wilf Keller
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  9. Denis J. Murphy
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  10. Laurian S. Robert
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Ping Hong, H., Ross, J.H., Gerster, J.L. et al. Promoter sequences from two different Brassica napus tapetal oleosin-like genes direct tapetal expression of β-glucuronidase in transgenic Brassica plants. Plant Mol Biol 34, 549–555 (1997). https://doi.org/10.1023/A:1005840824926

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  • DOI: https://doi.org/10.1023/A:1005840824926

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