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Characterization of 26S proteasome α- and β-type and ATPase subunits from spinach and their expression during early stages of seedling development

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Abstract

Three kinds of cDNAs encoding 26S proteasome subunits have been cloned from spinach (Spinacia oleracea L.). These genes, designated as SOPSC8, SOPSC1 and SOPRS7, encode an α-type and a β-type subunit of the 20S catalytic core, and an ATPase subunit of the 19/22S regulatory complex, respectively. The deduced protein sequences showed high sequence similarities to other proteasome α- and β-type and ATPase subunit proteins. Southern blot analysis indicates that there are additional members of these dispersed proteasome families in the spinach genome. These three subunit genes are expressed simultaneously during germination and reach a maximum one day after sowing followed by a decline. The expression of these genes also increases during cotyledon senescence.

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Author notes

  1. Ken-ichi Tomizawa

    Present address: Plant Molecular Physiology Laboratory, Research Institute of Innovative Technology for the Earth (RITE), Kizu-cho, Kyoto, 619-02, Japan

Authors and Affiliations

  1. Division of Bioproduction Science, Faculty of Horticulture, Chiba University, Matsudo 648, Matsudo, Chiba, 271, Japan

    Naoko Ito, Takahide Sato & Hiroki Nakagawa

  2. Laboratory for Photoperception and Signal Transduction, Frontier Research Program, The Institute of Physical and Chemical Research (RIKEN), Hirosawa 2-1, Wako, Saitama, 351-01, Japan

    Naoko Ito, Ken-ichi Tomizawa, Minami Matsui & Richard E. Kendrick

  3. Division of Chemotherapy, The Tokyo Metropolitan Institute of Medical Science, Hon-komagome 3-18-22, Bunkyo, Tokyo, 113, Japan

    Keiji Tanaka

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Ito, N., Tomizawa, Ki., Tanaka, K. et al. Characterization of 26S proteasome α- and β-type and ATPase subunits from spinach and their expression during early stages of seedling development. Plant Mol Biol 34, 307–316 (1997). https://doi.org/10.1023/A:1005839501822

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