Abstract
A non-viral transfection method for oral mucosal cells was investigated using a modified transfection method and five commercial transfection reagents. The CellFECTINTM gave the highest expression of a transfected gene. When the mucosal cells were transfected with 0.3 ng DNA/cell, the transfection efficiency was optimal, and the production of a reporter protein increased up to ten times higher than those with the other transfection reagents.
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Nagatani, N., Shinkai, M., Nagase, Y. et al. Gene delivery for genetically engineered mucosal cells with enhanced function. Biotechnology Letters 22, 999–1002 (2000). https://doi.org/10.1023/A:1005693321390
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DOI: https://doi.org/10.1023/A:1005693321390