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A dynamic membrane reactor with immobilized α-chymotrypsin for continuous kyotorphin synthesis in organic media

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Abstract

α-Chymotrypsin was covalently attached to an α-alumina ultrafiltration membrane coated with an inert protein. This derivative was used as catalyst for the continuous kinetically controlled synthesis of kyotorphin in both membrane and packed bed reactors, using aqueous (water/dimethyl sulfoxide, 60:40, v/v) and nearly-dry (hexane/ethanol/water, 57:40:3, by vol.) organic media, respectively. In both media, the synthetic activity and operational stability of the enzyme-membrane derivative was compared with an adsorbed α-chymotrypsin Celite derivative, being 2-times and 4-times respectively, higher than the Celite one. The enzyme-membrane derivative showed half-life times higher than 36 days, with a selectivity near to 100%.

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Authors and Affiliations

  1. Departamento de Bioquímica y Biología Molecular B e Inmunología, Facultad de Química, Universidad de Murcia, P.O. Box 4021, E-30100, Murcia, España

    P. Lozano, T. De Diego & J.L. Iborra

  2. Laboratoire de Matériaux et des Procédés Membranaires, LMPM UMR 5635, Université de Montpellier 2, CC 024, Place E. Bataillon, F-34095, Montpellier Cédex 05, France

    M.P. Belleville & G.M. Rios

Authors
  1. P. Lozano
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  2. T. De Diego
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  3. M.P. Belleville
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  4. G.M. Rios
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  5. J.L. Iborra
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Lozano, P., De Diego, T., Belleville, M. et al. A dynamic membrane reactor with immobilized α-chymotrypsin for continuous kyotorphin synthesis in organic media. Biotechnology Letters 22, 771–775 (2000). https://doi.org/10.1023/A:1005690604799

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  • DOI: https://doi.org/10.1023/A:1005690604799

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