Abstract
Mandelate racemase [EC 5.1.2.2] from Pseudomonas putida ATCC 12336 was efficiently immobilized through ionic binding onto DEAE- and TEAE 23-cellulose. The activity of the immobilized enzyme was significantly enhanced as compared to the native protein, i.e., 2.7- and 2.5-fold, respectively. DEAE-cellulose-immobilized mandelate racemase could be efficiently used in repeated batch reactions for the racemization of (R)-mandelic acid under mild conditions.
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Strauss, U.T., Kandelbauer, A. & Faber, K. Stabilization and activity-enhancement of mandelate racemase from Pseudomonas putida ATCC 12336 by immobilization. Biotechnology Letters 22, 515–520 (2000). https://doi.org/10.1023/A:1005621021983
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DOI: https://doi.org/10.1023/A:1005621021983