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Cloning and nucleotide sequence of a novel cry1Aa-type gene from Bacillus thuringiensis subsp. kurstaki

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Abstract

A newly isolated strain of B. thuringiensis, BNS3, was identified as affiliated to the subsp. kurstaki and belonging to the serotype H3a, 3b, 3c. Insecticidal crystal proteins from BNS3 were active against lepidopteran larvae, particularly Prays oleae, Ephestia kuehniella, Ostrinia nubilalis and Spodoptora exigua. The cloning and sequencing from BNS3 of a cry1Aa-type gene, called crybns3-1, revealed an open reading frame of 3531 bp, encoding a protein of 1176 amino acid residues. Both nucleotide and amino acid sequences similarity analysis revealed that crybns3-1 is a new cry1Aa-type gene, presenting several differences with the other cry1Aa-type genes.

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References

  • Carozzi NB, Kramer VC, Warren GW, Evola S, Koziel MG (1991) Prediction of insecticidal activity of Bacillus thuringiensis strains by polymerase chain reaction product profiles. Appl. Environ. Microbiol. 57: 3057–3061.

    Google Scholar 

  • Crickmore N, Zeigler DR, Feitelson J, Schnepf HE, Van Rie J, Lereclus D, Baum J, Dean DH (1998) Revision of the nomenclature for the Bacillus thuringiensis pesticidal crystal proteins. Microbiol. Mol. Biol. Rev. 62: 807–813.

    Google Scholar 

  • Dean DH, Rajamohan F, Lee MK, Wu SJ, Chen XJ, Alcantara E, Hussainn SR (1996) Probing the mechanism of action of Bacillus thuringiensis insecticidal proteins by site-directed mutagenesis. Gene 179: 111–117.

    Google Scholar 

  • Geiser M, Schweitzer S, Grimm C (1986) The hypervariable region in the genes coding for entomopathogenic crystal proteins of Bacillus thuringiensis: nucleotide sequence of the kurhd1 gene of subsp. kurstaki HD1. Gene 48: 109–118.

    Google Scholar 

  • Henikoff S (1984) Unidirectional digestion with exonuclease III creates targeted breakpoints for DNA sequencing. Gene 28: 351–359.

    Google Scholar 

  • Jaoua S, Zouari N, Tounsi S, Ellouz R (1996) Study of the δ-endotoxins produced by three recently isolated strains of Bacillus thuringiensis. FEMS Microbiol. Lett. 145: 349–354.

    Google Scholar 

  • Lereclus D, Arantès O, Chaufaux J, Lecadet MM (1989) Transformation and expression of a cloned δ-endotoxin gene in Bacillus thuringiensis. FEMS Microbiol. Lett. 60: 211–218.

    Google Scholar 

  • Masson L, Marcotte P, Préfontaine G, Brousseau R (1989) Nucleotide sequence of a gene cloned from Bacillus thuringiensis subsp. entomocidus coding for an insecticidal protein toxic for Bombyx mori. Nucleic Acids Res. 17: 446.

    Google Scholar 

  • Sambrook J, Fritsh EF, Maniatis T (1989) Molecular Cloning. A Laboratory Manual, 2nd edn. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory.

    Google Scholar 

  • Schnepf HE, Wong HC, Whitely HR (1985) The amino acid sequence of a crystal protein from Bacillus thuringiensis deduced from DNA base sequence. J. Biol. Chem. 260: 6264–6272.

    Google Scholar 

  • Schnepf HE, Crickmore N, Van Rie J, Lereclus D, Baum J, Feitelson J, Zeigler DR, Dean DH (1998) Bacillus thuringiensis and its pesticidal crystal proteins. Microbiol. Mol. Biol. Rev. 62: 775–806.

    Google Scholar 

  • Shimizu M, Oshie K, Nakamura K, Takada Y, Oeda K, Ohkawa H (1988) Cloning and expression in Escherichia coli of the 135 kDa insecticidal protein gene from Bacillus thuringiensis subsp. kurstaki IPL7. Agric. Biol. Chem. 52: 1565–1573.

    Google Scholar 

  • Shin BS, Park SH, Choi SK, Koo BT, Lee ST, Kim JI (1995) Distribution of cryV-type insecticidal protein genes in Bacillus thuringiensis and cloning of cryV-type genes from Bacillus thuringiensis subsp. kurstaki and Bacillus thuringiensis subsp. entomocidus. Appl. Environ. Microbiol. 61: 2402–2407.

    Google Scholar 

  • Southern EM (1975) Detection of specific sequences among DNA fragments separated by gel electrophoresis. J. Mol. Biol. 98: 503–517.

    Google Scholar 

  • Udayasuriyan V, Nakamura A, Mori H, Masaki H, Uozumi T (1994) Cloning of a new cryIA(a) gene from Bacillus thuringiensis strain FU–2–7 and analysis of chimeric Cry IA(a) proteins for toxicity. Biosci. Biotechnol. Biochem. 58: 830–835.

    Google Scholar 

  • Wong HC, Schnepf HE, Whiteley HR (1983) Transcriptional and translational start sites for the Bacillus thuringiensis crystal protein gene. J. Biol. Chem. 258: 1960–1967.

    Google Scholar 

  • Zouari N, Jaoua S (1997) Purification and immunological characterization of particular delta-endotoxins from three strains of Bacillus thuringiensis. Biotechnol. Lett. 19: 825–829.

    Google Scholar 

  • Zouari N, Dhouib A, Ellouz R, Jaoua S (1998) Nutritional requirements of a strain of Bacillus thuringiensis subsp. kurstaki and use of gruel hydrolyzate for the formulation of a new medium for delta-endotoxin production. Appl. Biochem. Biotechnol. 69: 41–52.

    Google Scholar 

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Authors and Affiliations

  1. Centre de Biotechnologie de Sfax, Laboratoire de Protection et de Transformation des Plantes, B.P. ‘K’, 3038, Sfax, Tunisia

    Slim Tounsi, Anis J'Mal, Nabil Zouari & Samir Jaoua

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  1. Slim Tounsi
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  2. Anis J'Mal
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  3. Nabil Zouari
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  4. Samir Jaoua
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Tounsi, S., J'Mal, A., Zouari, N. et al. Cloning and nucleotide sequence of a novel cry1Aa-type gene from Bacillus thuringiensis subsp. kurstaki . Biotechnology Letters 21, 771–775 (1999). https://doi.org/10.1023/A:1005579206907

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  • DOI: https://doi.org/10.1023/A:1005579206907

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