Abstract
Candida magnoliae and its mutants were analyzed to produce erythritol from glucose with high yield and productivity. One mutant, M2, showed higher erythritol conversion yield and productivity than the wild strain. The osmophilic mutant produced 25 g erythritol l−1 after 83 h of a flask culture in a medium containing 10% (w/v) glucose, corresponding to a 25% increase in erythritol and a 30% increase in erythritol productivity compared with the wild type. The fermentation properties were further improved by cultivating the osmophilic mutant in a fermenter containing 20% (w/v) glucose medium with 0.54 g l−1 h−1 of erythritol productivity and 43% of erythritol conversion yield based on glucose.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Aoki MAY, Pastore GM, Park YK (1993) Microbial transformation of sucrose and glucose to erythritol. Biotechnol. Lett. 15: 383–388.
Bornet F, Dauchy F, Chevalier A, Slama G (1992). Etude de devenir metabolique, après ingestion chez l'erythritol. Clin. Biol. 16: A169.
Chun YJ, Seo SH (1995) Development of erythritol production process by microbial fermentation. Kor. Biotechnol. Chem. Eng. 9: 24–29.
Han WO, Seo JH, Ryu YW (1998) Xylitol production by catabolite repressed mutant of Candida sp. Kor. J. Biotechnol. Bioeng. 13: 1–6
Hajny GJ, Smith JH, Garver JC (1964) Erythritol production by a yeastlike fungus. Appl. Microbiol. 12: 240–246.
Ishizuka H, Wako K, Kasumi T, Sasaki T (1989) Breeding of a mutant of Aureobasidium sp. with high erythritol production. J. Ferment. Bioeng. 68: 310–314.
Kim KA, Noh BS, Kim SY, Oh DK (1999) Effect of osmotic pressure of salts on growth of Torula sp. and erythritol production. Kor. J. Appl. Microbiol. Biotechnol. 27: 91–95.
Kim SY, Park SS, Chun YJ, Seo JH (1996) Analysis of erythritol fermentation properties by Candida sp. Kor. J. Food Sci. Technol. 28: 935–939.
Kawanabe J, Hirasawa M, Takeuchi T, Oda T, Ikeda T (1992) Noncariogenicity of erythritol as a substrate. Caries Res. 26: 358–362.
Park JB, Seo BC, Kim JR, Pek UH, Park YK (1998) Effect of glucose concentration on the production of erythritol by Trichosporon sp. J. Microbiol. Biotechnol. 8: 543–546.
Park JB, Seo BC, Kim JR, Park YK (1998) Production of erythritol in fed-batch cultures of Trichosporon sp. J. Ferment. Bioeng. 86: 577–580.
Roper H, Goossens J (1993) Erythritol, a new material for food and non-food applications. Starch/Starke 45: 400–405.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Yang, SW., Park, JB., Soo Han, N. et al. Production of erythritol from glucose by an osmophilic mutant of Candida magnoliae . Biotechnology Letters 21, 887–890 (1999). https://doi.org/10.1023/A:1005566420982
Issue Date:
DOI: https://doi.org/10.1023/A:1005566420982