Abstract
Linkage of random amplified polymorphic DNA (RAPD) markers with resistance genes to clubroot (Plasmodiophora brassicae Wor.) in Brassica rapa L. was studied in a doubled haploid (DH population obtained by microspore culture. Thirty-six DH lines were obtained from F1 plants from a cross between susceptible ‘Homei P09’ and resistant ‘Siloga S2’ plants. ‘Homei P09’ was a DH line obtained by microspore culture of the Chinese cabbage variety ‘Homei’, which is highly responsive in microspore culture. The resistant line ‘Siloga S2’ was obtained by two rounds of selfing of the fodder turnip ‘Siloga’. Three RAPD markers, RA12-75A, WE22B and WE49B, were found to be linked to a clubroot-resistance locus. These three markers were linked in the DH lines and an F2 population and should be useful for marker-assisted selection in breeding programs.
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Ajisaka, H., Y. Kuginuki, K. Hida, S. Enomoto & M. Hirai, 1995. A linkage map of DNA markers in Brassica campestris. Breed Sci 45(Suppl. 2): 195 (in Japanese).
Buczacki, S.T., H. Toxopeus, P. Mattusch, T.D. Johnston, G.R. Dixon & L.A. Hobolth, 1975. Study of physiologic specialization in Plasmodiophora brassicae: Proposals for attempted rationalization through an international approach. Trans Br Mycol Soc 65: 295–303.
Crute, I.R., A.R. Gray, P. Crisp & S.T. Buczacki, 1980. Variation in Plasmodiophora brassicae and resistance to clubroot disease in brassicas and allied crops — a critical review. Plant Breed Abst 50: 91–104.
Edwards, K., C. Johnstone & C. Thompson, 1991. A simple and rapid method for the preparation of plant genomic DNA for PCR analysis. Nucleic Acids Res 19: 1349.
Figdore, S.S., M.E. Ferreira, M.K. Slocum & P.H. Williams, 1993. Association of RFLP markers with trait loci affecting clubroot resistance and morphological characters in Brassica oleracea L. Euphytica 69: 33–44.
Grandclement, C. & G. Thomas, 1996. Detection and analysis of QTLs based on RAPD markers for polygenic resistance to Plasmodiophora brassicae Woron in Brassica oleracea L. Theor Appl Genet 93: 86–90.
Hamilton, H.A. & R. Crete, 1978. Influence of soil moisture, soil pH, and liming sources on the incidence of clubroot, the germination and growth of cabbage produced in mineral and organic soils under controlled conditions. Can J Plant Sci 58: 45–53.
Hashizume, T., T. Sato & M. Hirai, 1993. Determination of genetic purity of hybrid seed in watermelon (Citrullus lanatus) and tomato (Lycopersicon esculentum) using random amplified polymorphic DNA (RAPD). Japan J Breed 43: 367–375.
Jones, D.R., D.S. Ingram & G.R. Dixon, 1982. Characterization of isolates derived from single resting spores of Plasmodiophora brassicae and studies of their interaction. Plant Pathol 31: 239–246.
Karling, J.S., 1968. The Plasmodiophorales. Hafner Publ Co, New York. 239 pp.
Kuginuki, Y., H. Yoshikawa & K. Hida, 1994. Breeding of Chinese cabbage with clubroot resistance in Japan. In: ISHS Symposium on Brassicas, Abstracts of 9th Crucifer Genetics Workshop, Lisbon, pp. 15.
Landry, B.S., N. Hubert, R. Crete, M.S. Chiang, S.E. Lincoln & T. Etoh, 1992. A genetic map for Brassica oleracea based on RFLP markers detected with expressed DNA sequences and mapping of resistance genes to race 2 of Plasmodiophora brassicae (Woronin). Genome 35: 409–420.
Murashige, T. & F. Skoog, 1962. A revised medium for rapid growth and bio assays with tobacco tissue cultures. Physiol Plant 15: 473–497.
Murray, M. & W.F. Thompson, 1980. Rapid isolation of high molecular weight plant DNA. Nucleic Acids Res 8: 4321–4325.
Pink, D.A.C., G.J. King, D.J. Ockendon, R. Mclenaghan & J. Lynn, 1994. Use of anther culture and molecular markers in breeding for resistance to Plasmodiophora brassicae. In: ISHS Symposium on Brassicas, Abstracts of 9th Crucifer Genetics Workshop, Lisbon. pp. 15.
Sato, T., T. Nishio & M. Hirai, 1989. Plant regeneration from isolated microspore cultures of Chinese cabbage (Brassica campestris ssp. pekinensis). Plant Cell Reports 8: 486–488.
U, N., 1935. Genome-analysis in Brassica with special reference to the experimental formation of B. napus and peculiar mode of fertilization. Japan J Bot 7: 389–452.
Yoshikawa, H., 1981. Breeding for clubroot resistance in Chinese cabbage. In: N.S. Talekar & T.D. Griggs (Eds.), Chinese Cabbage, pp. 405–413. Proceedings of the 1st International Symposium, Tsukuba, Japan.
Voorrips, R.E., 1995. Plasmodiophora brassicae: Aspects of pathogenesis and resistance in Brassica oleracea. Euphytica 83: 139–146.
Wilcoxon, F., 1945. Individual comparisons by ranking methods. Biometrics 1: 80–83.
Williams, P.H., 1966. A system for the determination of races of Plasmodiophora brassicae that infect cabbage and rutabaga. Phytopathology 56: 624–626.
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Kuginuki, Y., Ajisaka, H., Yui, M. et al. RAPD markers linked to a clubroot-resistance locus in Brassica rapa L.. Euphytica 98, 149–154 (1997). https://doi.org/10.1023/A:1003147815692
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DOI: https://doi.org/10.1023/A:1003147815692