biologia plantarum

International journal on Plant Life established by Bohumil Nìmec in 1959

Biologia plantarum 43:217-220, 2000 | DOI: 10.1023/A:1002796009329

Introduction of Alcohol Dehydrogenase in Lettuce Seedlings by Flooding Stress

H. Kato-Noguchi1, H. Saito1
1 Department of Biochemistry and Food Science, Faculty of Agriculture, Kagawa University, Miki, Kagawa, Japan

Alcohol dehydrogenase (ADH), its isozyme profiles and ethanol concentration in lettuce (Lactuca sativa L.) seedlings subjected to flooding stress were determined. Flooding stress caused increases in ADH activity and ethanol concentration. By 48 h, ADH activity and ethanol concentration in the flooded seedlings increased 3.2- and 7.0-fold, respectively, in comparison with those in non-stressed seedlings. Five electrophoretically separable ADH bands were found in extract of the flooded seedlings, whereas only two or three ADH bands were found in extract of non-stressed seedlings. These results indicate that lettuce ADH may have a system of three-gene and six-isozyme, and the increase in ADH activity in the flooded seedlings may be due to increased synthesis of the enzyme.

Keywords: Lactuca sativa; ethanol; isozymes; low oxygen; waterlogging
Subjects: alcohol dehydrogenase, flooding stress; ethanol, flooding stress; flooding stress, induction of alcohol dehydrogenase; Lactuca sativa; lettuce, alcohol dehydrogenase, flooding; oxygen, low concentration, alcohol dehydrogenase; waterlogging, alcohol dehydrogenase

Published: August 1, 2000  Show citation

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Kato-Noguchi, H., & Saito, H. (2000). Introduction of Alcohol Dehydrogenase in Lettuce Seedlings by Flooding Stress. Biologia plantarum43(2), 217-220. doi: 10.1023/A:1002796009329
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References

  1. Bradford, M.M.: A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding.-Anal. Biochem. 72: 248-254, 1976. Go to original source...
  2. Chourey, P., Widholm, J.: Tissue specific alcohol dehydrogenase isozyme variation in carrot: Whole plant versus in vitro cultured cells.-In Vitro 16: 571-574, 1980. Go to original source...
  3. Crawford, R.M.M.: Physiological responses to flooding.-In: Lange, O.L., Nobel, P.S., Osmond, C.B., Ziegler, H. (ed.): Physiological Plant Ecology II. Water Relations and Carbon Assimilation. Pp. 453-477. Springer-Verlag, New York 1982. Go to original source...
  4. Davies, D.D.: Anaerobic metabolism and the production of organic acids.-In: Davies, D.D. (ed.): The Biochemistry of Plants. Vol. 2. Pp. 581-611. Academic Press, New York 1980. Go to original source...
  5. Drew, M.C.: Oxygen deficiency and root metabolism: Injury and acclimation under hypoxia and anoxia.-Annu. Rev. Plant. Physiol. Plant. mol. Biol. 48: 223-250, 1997. Go to original source...
  6. Good, A.G., Crosby, W.L.: Anaerobic induction of alanine aminotransferase in barley root tissue.-Plant Physiol. 90: 1305-1309, 1989. Go to original source...
  7. Gottlieb, L.P.: Conservation and duplication of isozymes in plants.-Science 216: 373-380, 1982. Go to original source...
  8. Hanson, A.D., Jacobsen, J.V., Zwar, J.A.: Regulated expression of three alcohol dehydrogenase genes in barely alcurone layers.-Plant Physiol. 75: 573-581, 1984. Go to original source...
  9. Kato-Noguchi, H., Watada, A.E.: Effects of low-oxygen atmosphere on ethanolic fermentation in fresh-cut carrots.-J. amer. Soc. hort. Sci. 122: 107-111, 1997. Go to original source...
  10. Kato-Noguchi, H.: Flooding induced increases in alcohol dehydrogenase acivity in timothy and ryegrass seedlings.-Biol. Plant. 42: 445-449, 1999. Go to original source...
  11. Ke, D., Yahia, E., Mateos, M., Kader, A.A.: Ethanolic fermentation of Bartlett' pears as influenced by ripening stage and atmospheric composition.-J. amer. Soc. hort. Sci. 119: 976-982, 1994. Go to original source...
  12. Ke, D., Yahia, E., Hess, B., Zhou, L., Kader, A.A.: Regulation of fermentative metabolism in avocado fruit under oxygen and carbon dioxide stresses.-J. amer. Soc. hort. Sci. 120: 481-490, 1995. Go to original source...
  13. Kennedy, R.A., Rumpho, M.E., Fox, T.C.: Anacrobic metabolism in plants.-Plant Physiol. 100: 1-6, 1992. Go to original source...
  14. Muench, D.G., Archibold, O.W., Good, A.G.: Hypoxic metabolism in wild rice (Zizania palustris): Enzyme induction and metabolite production.-Physiol. Plant. 89: 165-171, 1993. Go to original source...
  15. Newman, K.D., VanToai, T.T.: Cell biology and molecular genetics: Developmental regulation and organ-specific expression of soybean alcohol dehydrogenase.-Crop Sci. 31: 1253-1257, 1991. Go to original source...
  16. Paul, A.-L., Ferl, R.J.: In vivo footprinting reveals unique cis-elements and different models of hypoxic induction in maize Adh1 and Adh2.-Plant Cell 3: 159-168, 1991. Go to original source...
  17. Ricard, B., Couĉe, I., Raymond, P., Saglio, P.H., Saint-Ges, V., Pradet, A.: Plant metabolism under hypoxia and anoxia.-Plant Physiol. Biochem. 32: 1-10, 1994.
  18. Roberts, J.K.M., Callis, J., Wemmer, D., Walbot, V., Jardetzky, O.: Mechanism of cytoplasmic pH regulation in hypoxic maize roots tips and its role in survival under hypoxia.-Proc. nat. Acad. Sci. USA 81: 3379-3383, 1984. Go to original source...
  19. Sachs, M.M., Ho, T.-H.D.: Alteration of gene expression during environmental stress in plants.-Annu. Rev. Plant Physiol. 37: 363-376, 1986. Go to original source...
  20. Vartapetian, B.B., Jackson, M.B.: Plant adaptations to anaerobic stress.-Ann. Bot. 79: 2-20, 1997. Go to original source...
  21. Waters, I., Morrell, S., Greenway, H., Colmer, T.D.: Effects of anoxia on wheat seedlings. II. Influence of O2 supply prior to anoxia on tolerance to anoxia, alcoholic fermentation, and sugar levels.-J. exp. Bot. 42: 1437-1447, 1991. Go to original source...
  22. Xie, Y., Wu, R.: Rice alcohol dehydrogenase genes: Anaerobic induction, organ specific expression and characterization of cDNA clones.-Plant mol. Biol. 13: 53-68, 1989. Go to original source...