biologia plantarum

Biologia plantarum 39:183-192, 1997 | DOI: 10.1023/A:1001056201052

RAPD fingerprinting of diploid Lolium perenne × hexaploid Festuca arundinacea hybrid genomes

G. Šiffelová¹, M. Pavelková¹, A. Klabouchová¹, I. Wiesner¹, V. Našinec¹, I. Našinec²

¹ Academy of Sciences of the Czech Republic, Branišovská 31, České, Institute of Plant Molecular Biology, Budějovice, Czech Republic

² Grass Breeding Station, Oseva UNI - Choceň, Větrov, Czech Republic

We tested the application of RAPD technology for identification of hybrid genomes originated from a maternal clone of Lolium perenne L. (2n = 2x = 14) bearing cytoplasmic male sterility, which was pollinated separately by five clones of Festuca arundinacea Schreb. cv. Barocco (2n = 6x = 42). Six classes of RAPD markers were recognized, specific to: 1) Festuca genome and inherited into F1 hybrid genomes, 2) Lolium genome inherited into F1 hybrid genomes, 3) Lolium-specific bands not found in F1 progeny, 4) Festuca-specific bands not found in F1 progeny, 5) new bands found only in F1 hybrid profiles, 6) bands common to all parental and F1 hybrid genotypes. RAPD data were shown to have full potential a) to serve as an unequivocal proof of genome recombination in perennial ryegrass × tall fescue hybrids, b) to identify hybrid genomes, c) to reveal phenetic relationships of the accessions from crossing families, d) to enhance, by fingerprinting, the selection of superior hybrid material for further breeding. RAPD data were found to be consistent with the festucoid phenotype of F1 hybrids.

Keywords: cluster analysis; DNA typing; fescue; ryegrass

Subjects: cluster analysis; DNA typing; fescue × ryegrass hybrid; Festuca arundinacea; hybrid Lolium × Festuca, RAPD fingerprinting; Lolium perenne; random amplified polymorphic DNA; ryegrass × fescue hybrid

Published: September 1, 1997  Show citation