biologia plantarum

International journal on Plant Life established by Bohumil Němec in 1959

Biologia plantarum 39:245-250, 1997 | DOI: 10.1023/A:1001020720616

Biochemical changes in sugar beet lines in dependence on soil moisture

S. Kevrešan1, B. Krstić2, M. Popović1, L. Kovačev3, S. Pajević2, J. Kandrač1, Đ. Malenčić1
1 Faculty of Agriculture, University of Novi Sad, Trg Dositeja Obradovića 8, Novi Sad, Yugoslavia
2 Faculty of Sciences, University of Novi Sad, Trg Dositeja Obradovića 8, Novi Sad, Yugoslavia
3 Institute of Field and Vegetable Crops, Maksima Gorkog 30, Novi Sad, Yugoslavia

Nine sugar beet lines were grown in a glasshouse on chernozem soil watered to 35, 50 and 65 % of maximal soil water capacity. After 12 d, plant water potential, net photosynthetic rate, contents of soluble proteins, DNA and RNA, proportion of ribulose-1,5-bisphosphate carboxylase/oxygenase (RUBISCO) protein, and carbonic anhydrase activity were measured. As soil moisture decreased, the leaf water potential and net photosynthetic rate decreased. DNA and RNA content and carbonic anhydrase activity decreased under moderate drought, and increased with severe drought. RUBISCO protein proportion did not change significantly under decreased soil moisture, while the total soluble protein content decreased.

Keywords: Beta vulgaris L.; drought; DNA and RNA content; carbonic anhydrase activity; RUBISCO; soluble protein content
Subjects: Beta vulgaris; carbonic anhydrase, soil moisture; drought, photosynthesis; nucleic acids, soil moisture; ribulose-1,5-bisphosphate carboxylase/oxygenase, soil moisture; sugar beet lines, soil moisture

Published: September 1, 1997  Show citation

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Kevrešan, S., Krstić, B., Popović, M., Kovačev, L., Pajević, S., Kandrač, J., & Malenčić, Đ. (1997). Biochemical changes in sugar beet lines in dependence on soil moisture. Biologia plantarum39(2), 245-250. doi: 10.1023/A:1001020720616
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References

  1. Aliev, J.A., Guliev, N.M., Kerimov, S.K., Hidayatov, R.B.: Photosynthetic enzymes of wheat genotypes differing in productivity.-Photosynthetica 32: 77-85, 1996.
  2. Bradford, M.M.: A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding.-Anal. Biochem. 72: 248-254, 1976. Go to original source...
  3. Cherry, J.H.: Molecular Biology of Plants.-Columbia University Press, New York 1973.
  4. Damerval, C., de Vienne, D., Zivy, M., Thiellement, T.: Technical improvements in two-dimensional electrophoresis increase the level of genetic variation detected in wheat-seedling proteins.-Electrophoresis 7: 52-54, 1986. Go to original source...
  5. Dreesmann, D.C., Harn, C., Daie, J.: Expression of genes encoding RUBISCO in sugar beet (Beta vulgaris L.) plants subjected to gradual desiccation.-Plant Cell Physiol. 35: 645-653, 1994. Go to original source...
  6. Fredeen, A.L., Gamon, J.A., Field, C.O.: Response of photosynthesis and carbohydrate partitioning to limitations in nitrogen and water availability in field grown sunflower.-Plant Cell Environ. 14: 963-970, 1991. Go to original source...
  7. Fridlyand, L.E., Kaler, V.L.: Possible CO2 concentrating mechanism in chloroplasts of C-3 plants - role of carbonic anhydrase.-Gen. Physiol. Biophys. 6: 617-636, 1987.
  8. Kanechi, M., Kunitomo, E., Inagaki, N., Maekawa, S.: Water stress effects on ribulose-1,5-bisphosphate carboxylase and the relationship to photosynthesis in sunflower leaves.-In: Mathis, P. (ed.): Photosynthesis: from Light to Biosphere. Vol IV. Pp. 597-600. Kluwer Academic Publishers, Dordrecht-Boston-London 1995. Go to original source...
  9. Keys, A.J., Parry, M.A.J.: Ribulose bisphosphate carboxylase/oxygenase and carbonic anhydrase.-In: Dey, P.M., Harborne, J.B. (ed.): Methods in Plant Biochemistry. Vol. 3. Pp. 1-14. Academic Press, London 1990. Go to original source...
  10. Kruger, G.H.J., Van Rensburg, L.: Carbon dioxide fixation: stomatal and non-stomatal limitations in drought-stressed Nicotiana tabacum L. cultivars.-In: Mathis, P. (ed.): Photosynthesis: from Light to Biosphere. Vol IV. Pp. 505-510, Kluwer Academic Publishers, Dordrecht-Boston-London 1995. Go to original source...
  11. Laemmly, U.K.: Cleavage of structural proteins during the assembly of the head of bacteriophage T4.-Nature 227: 680-685, 1970. Go to original source...
  12. Lowry, O.H., Rosebrough, N.J., Farr, A.L., Randal, R.J.: Protein measurement with the Folin phenol reagent.-J. biol. Chem. 193: 265-275, 1951. Go to original source...
  13. Maaloee, O.: Regulation of the protein-synthesizing machinery-ribosomes, tRNA, factors, and so on,-Biol. Regul. Dev. 1: 487-542, 1979. Go to original source...
  14. Martin, B., Ruiz-Torres, H.A.: Effects of water-deficit stress on photosynthesis, its components and component limitations, and on water use efficiency in wheat (Triticum aestivum L.).-Plant Physiol. 100: 733-739, 1992. Go to original source...
  15. Pankovié, D.: [Photosynthesis in sunflower (Helianthus annuus L.) leaves under conditions of water deficit].-Ph.D. Thesis. Faculty of Biology, University of Belgrade, Belgrade 1996. [In Serbian.]
  16. Peltier, G., Cournac, L., Despax, V., Dimon, B., Fina, L., Genty, B., Rumcau, D.: Carbonic anhydrase activity in leaves as measured in vivo by 18O exchange between carbon-dioxide and water.-Planta 196: 732-739, 1995. Go to original source...
  17. Popova, L.P., Tsonev, T.D., Lazova, G.N., Stoinova, Z.G.: Drought-induced and ABA-induced changes in photosynthesis of barley plants.-Physiol. Plant. 96: 623-629, 1996. Go to original source...
  18. Raggi, V.: Changes in free amino acids and osmotic adjustment in leaves of water stressed bean.-Biol. Plant. 91: 427-434, 1995. Go to original source...
  19. Rickli, E.E., Ghazanfar, S.A.S., Gibbson, B., Edsall, J.T.: Carbonic anhydrases from human erythrocytes.-J. biol. Chem. 239: 1065-1078, 1964. Go to original source...
  20. Spirin, A.S. [Spectrophotometric determination of total nucleic acids.]-Biokhimiya 23: 656-662, 1958. [In Russ.]
  21. Walker, D.A.: Automated measurement of leaf photosynthetic O2 evolution as a function of photon flux density.-Phil. Trans. roy. Soc. Lond. B 323: 313-326, 1987. Go to original source...