biologia plantarum

International journal on Plant Life established by Bohumil Němec in 1959

Biologia plantarum 39:215-221, 1997 | DOI: 10.1023/A:1000388719570

Oxidative stress injury in tomato plants induced by supplemental UV-B radiation

T. Balakumar1, B. Gayathri1, P.R. Anbudurai1
1 Unit of Stress Physiology and Plant Biochemistry, Department of Botany, the American College, Madurai, India

Tomato (Lycopersicon esculentum Mill. cv. PKM 1) plants growing under field conditions were exposed for 15 d to solar radiation with UV-B component (280 - 320 nm) enhanced to 6.3 kJ m-2 d-1. This simulated a 15% stratospheric ozone depletion over Madurai (9° 50' N latitude). Lipid peroxidation in the leaves of UV-B treated plants was 32% higher compared to the control. Superoxide dismutase (SOD) and catalase activities registered parallel promotion by 126 and 50 %, respectively, in the UV-B treated plants. Further, the contents of total phenols and anthocyanins in the leaves have also been enhanced by 40 and 156%, respectively. On the contrary, polyphenol oxidase activity demonstrated a 58 % inhibition in the leaves of UV-B treated plants. While anthocyanins and phenols are proposed to act as antioxidants, the reduction in polyphenol oxidase activity may maintain the turnover of phenols in the UV-B treated plants.

Keywords: lipid peroxidation; oxidative stress; phenols; superoxide dismutase

Prepublished online: March 1, 1997; Published: September 1, 1997  Show citation

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Balakumar, T., Gayathri, B., & Anbudurai, P.R. (1997). Oxidative stress injury in tomato plants induced by supplemental UV-B radiation. Biologia plantarum, 39(2), 215-221. doi: 10.1023/A:1000388719570
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    References

    1. Balakumar, T.: Biochemical responses of cowpea (Vigna unguiculata (L.) Walp.) seedlings to UV-B irradiation with special reference to nitrogen metabolism.-Ph.D. Thesis, Madurai Kamaraj University, Madurai 1992.
    2. Balakumar, T., Hanibabu Vincent, V., Paliwal, K.: On the interaction of UV-B radiation (280-315 nm) with water stress in crop plants.-Physiol. Plant. 87: 217-222, 1993. Go to original source...
    3. Biggs, R.H., Kossuth, S.V., Teramura, A.H.: Response of 19 cultivars of soybean to UV-B irradiance.-Physiol. Plant. 53: 19-26, 1981. Go to original source...
    4. Bradford, M.M.: A rapid and sensitive method for the quantification of microgram quantities of protein utilizing the principle of protein-dye binding.-Anal. Biochem. 72: 248-254, 1976. Go to original source...
    5. Burke, J.J., Gamble, P.E., Hatfield, J.L., Quisenberry, J.F.: Plant morphological and biochemical responses to field water deficits. I. Response of glutathionc reductase activity and paraquat sensitivity.-Plant Physiol. 79: 415-419, 1985. Go to original source...
    6. Caldwell, M.M.: Solar UV irradiation and the growth and development of higher plants.-In: Giese, A.C. (ed.): Photophysiology. Vol. VI. Pp. 131-268. Academic Press, New York 1971. Go to original source...
    7. Caldwell, M.M., Teramura, A.H., Tevini, M.: The changing solar ultraviolet climate and the ecological consequences for higher plants.-Trees 4: 363-366, 1989. Go to original source...
    8. Dhindsa, R.S., Dhindsa, P.P., Tharpe, T.A.: Leaf senescence correlated with increased levels of membrane permeability and lipid peroxidation and decreased levels of superoxide dismutase and catalase.-J. exp. Bot. 23: 93-101, 1981. Go to original source...
    9. Dohler, G.: Effect of UV-B (280-320 nm) radiation on the 15N-nitrate assimilation of some algae.-Plant Physiol. (Life Sci. Adv.) 7: 79-84, 1988.
    10. Harborne, J.B.: Phytochemical Methods.-Chapman and Hall, London 1984. Go to original source...
    11. Jordan, B.R., He, J., Chow, W.S.: Changes in mRNA levels and peptide subunits of ribulose-1,5-bisphosphate carboxylase oxygenase in response to supplementary UV-B radiation.-Plant Cell Environ. 15: 91-98, 1992. Go to original source...
    12. Kar, M., Misra, D.: Catalase, peroxidase and polyphenol oxidase activities during rice leaf senescence.-Plant Physiol. 57: 315-319, 1976. Go to original source...
    13. Knox, J.P., Dodge, A.D.: Singlet oxygen and plants.-Phytochemistry 24: 889-896, 1985. Go to original source...
    14. Kramer, G.F., Norman, H.A., Krizek, D.T., Mirecki, R.: Influence of UV-B radiation on polyamines, lipid peroxidation and membrane lipids in cucumbers.-Phytochemistry 30: 2101-2108, 1991. Go to original source...
    15. Larson, R.A.: The antioxidants of higher plants.-Phytochemistry 27: 969-978, 1988. Go to original source...
    16. Mancinelli, A.L., Walsh, L.: Photocontrol of anthocyanin synthesis. VII. Factors affecting the spectral sensitivity of anthocyanin synthesis in young seedlings.-Plant Physiol. 63: 841-846, 1978. Go to original source...
    17. Monk, L.S., Fagerstedt, K.V., Crawford, R.M.M.: Oxygen toxicity and superoxide dismutase as an antioxidant in physiological stress.-Physiol. Plant. 76: 456-459, 1989. Go to original source...
    18. Mukherjee, P.K., Ghosh, J.J.: Polyphenol oxidase activity in relation to resistance of rice to infection by Helminthosporium oryzae.-Sci. Cult. 41: 433-435, 1975.
    19. Nedunchezhian, N., Kulandaivelu, G.: Evidence for the ultraviolet-B (280-320 nm) radiation induced structural reorganization and damage of photosystem II polypeptides in isolated chloroplasts.-Physiol. Plant. 81: 558-562, 1991. Go to original source...
    20. Noorudeen, A.M., Kulandaivelu, G.: On the possible site of inhibition of photosynthetic electron transport by ultraviolet-B radiation.-Physiol. Plant. 55: 161-166, 1982. Go to original source...
    21. Panagopoulus, I., Bornman, J.F., Bjorn, L.O.: Effects of ultraviolet radiation and visible light on growth, fluorescence induction, ultraweak luminescence and peroxidase activity in sugar beet plants.-J. Photochem. Photobiol. B 8: 73-87, 1990. Go to original source...
    22. Renger, G., Volker, M., Eckert, H.J., Fromme, R., Hohm-Veit, S., Graber, P.: On the mechanism of photosystem II deterioration by UV-B radiation.-Photochem. Photobiol. 49: 97-105, 1989. Go to original source...
    23. Robbrecht, R., Caldwell, M.M.: Leaf UV optical properties of Rumex patientia L. and Rumex obtusifolius L. in regard to a protective mechanism against solar UV-B radiation injury.-In: Worrest, R.C., Caldwell, M. (ed.): Stratospheric Ozone Reduction, Solar Ultraviolet Radiation and Plant Life. Pp. 251-259 Springer-Verlag, Berlin-Heidelberg-New York 1986. Go to original source...
    24. Sawada, J., Ohyama, T., Yamazaki, I.: Preparation and physicochemical properties of green pea superoxide dismutase.-Biochem. biophys. Acta 268: 305-312, 1972. Go to original source...
    25. Stepleton, E.: Ultraviolet radiation and plants: Burning questions.-Plant Cell 4: 1353-1358, 1992. Go to original source...
    26. Tevini, M.: Stratospheric Ozone Depletion and its Effects on Plants.-Pergamon Press, New York 1993.
    27. Tevini, M., Teramura, A.H.: UV-B effects on terrestrial plants.-Photochem. Photobiol. 50: 479-487, 1989. Go to original source...
    28. Witztum, A., Keven, O., Even-Chem, Z.: The effect of UV radiation and sucrose on IAA levels in Spirodela oligorrhiza.-Ann. Bot. 42: 595-598, 1978. Go to original source...
    29. Zaprometov, M.N., Zagoskina, N.V.: [Further evidence for chloroplast participation in biosynthesis of phenolic compounds.]-Fiziol. Rast. 34: 165-171, 1987. [In Russ.]

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