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Improved low pH bicelle system for orienting macromolecules over a wide temperature range

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Abstract

We have prepared and characterized a novel bicelle system composed of 1,2-di-O-dodecyl-sn-glycero-3-phos- phocholine (DIODPC) and 3-(chloramidopropyl)dimethylammonio-2-hydroxyl-1-propane sulfonate (CHAPSO). At the optimal DIODPC/CHAPSO molar ratio of 4.3:1, this medium becomes magnetically oriented from pH 6.5 down to pH 1.0. Unlike previously reported bicelle preparations, these bicelles are chemically stable at low pH and are capable of inducing protein alignment, as illustrated by the large residual dipolar couplings measured for rusticyanin from Thiobacillus ferrooxidans at pH 2.1. The DIODPC/CHAPSO system is particularly useful for measuring residual dipolar couplings of macromolecules that require very acidic conditions.

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Cavagnero, S., Dyson, H.J. & Wright, P.E. Improved low pH bicelle system for orienting macromolecules over a wide temperature range. J Biomol NMR 13, 387–391 (1999). https://doi.org/10.1023/A:1008360022444

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  • DOI: https://doi.org/10.1023/A:1008360022444

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