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Clinical Correlation of Cyclin D2 mRNA Expression in Human Luteinized Granulosa Cells

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Abstract

Purpose: The aim was to evaluate whether the expression of cyclin D2 mRNA in human luteinized granulosa cells correlates with the clinical parameters of women undergoing in vitro fertilization–embryo transfer (IVF-ET) programs.

Methods: Using reverse transcriptase–polymerase chain reaction (RT-PCR) techniques, the cyclin D2 mRNA in human luteinized granulosa cells of large follicles was measured. Correlation analysis between cyclin D2 mRNA expression and clinical parameters [age; day3 follicle-stimulating hormone (FSH), luteinizing hormone (LH), and estradiol (E2); E2 and P4 at the day of human chorionic gonadotropin (hCG); total amount of gonadotropin and the number of days of gonadotropin administration; pregnancy outcome] were performed.

Result(s): Cyclin D2 mRNA expression showed negative correlation with patient's age (R = −0.60, P < 0.005) and total amount of gonadotropins (R = −0.45, P < 0.03), and also showed positive correlation with serum E2 levels at the day of hCG administration (R = 0.55, P < 0.02). Other clinical parameters showed no statistical significance. Cyclin D2 mRNA expression of pregnant group was higher than that of nonpregnant group (P < 0.03).

Conclusions: Cyclin D2 mRNA expression in human luteinized granulosa cells positively correlates with clinical parameters of ovarian function and may be a novel marker for the health of human ovarian follicle.

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REFERENCES

  1. Seifer DB, Gardiner AC, Ferreira KA: Apoptosis as a function of ovarian reserve in women undergoing in vitro fertilization. Fertil Steril 1996;66:593-598

    Google Scholar 

  2. Nakahara K, Saito H, Saito T: Incidence of apoptotic bodies in membrana granulosa of the patients participating in an in vitro fertilization program. Fertil Steril 1997;67:302-308

    Google Scholar 

  3. Nakahara K, Saito H, Saito T: The incidence of apoptotic bodies in membrana granulosa can predict prognosis of ova from patients participating in vitro fertilization programs. Fertil Steril 1997;68:312-317

    Google Scholar 

  4. Giudice LC: Insulin-like growth factors and ovarian follicular development. Endocrinol Rev 1992;13:641-669

    Google Scholar 

  5. Chun SY, Billig H, Tilly JL: Gonadotropin suppression of apoptosis in cultured preovulatory follicles: Mediatory role of endogenous insulin-like growth factor I. Endocrinology 1994;135:1845-1853

    Google Scholar 

  6. Robker RL, Richards JS: Hormone-induced proliferation and differentiation of granulosa cells: A coordinated balance of the cell cyclin regulators cyclin D2 and p27KIP1. Mol Endocrinol 1998;12:924-940

    Google Scholar 

  7. Sicinski P, Donaher JL, Geng Y: Cyclin D2 is an FSH-respective gene involved in gonadal cell proliferation and oncogenesis. Nature 1996;384:470-474

    Google Scholar 

  8. Dirnfeld M, Goldman S, Yael G: Asimplified coculture system with luteinzed granulosa cells improves embryo quality and implantation rates: A controlled study. Fertil Steril 1997;67:120-122

    Google Scholar 

  9. Sherr CJ: Cancer cell cycles. Science 1996;274:1672-1677

    Google Scholar 

  10. Inaba T, Matsushim H, Valentine M: Genomic organization, chromosomal localization, and independent expression of human cyclin D genes. Genomics 1992;13:565-574

    Google Scholar 

  11. Xiong Y, Menninger J, Beach D: Molecular cloning and chromosomal mapping of CCND genes encoding human D-type cyclins. Genomics 1992;13:575-584

    Google Scholar 

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Choi, D., Lee, E.Y., Yoon, S. et al. Clinical Correlation of Cyclin D2 mRNA Expression in Human Luteinized Granulosa Cells. J Assist Reprod Genet 17, 574–579 (2000). https://doi.org/10.1023/A:1026470825514

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  • DOI: https://doi.org/10.1023/A:1026470825514

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