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Human Umbilical Vein Endothelial Cells Express Complement Receptor 1 (CD35) and Complement Receptor 4 (CD11c/CD18) in vitro

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Abstract

We have examined complement receptors on human umbilical vein endothelial cells (HUVEC) and found that they express complement receptor 1 (CR1, CD35) and complement receptor 4 (CR4, CD11c/CD18), but not complement receptor 3 (CR3, CD11b/CD18). Binding of monoclonal antibodies against CR1 (CD35) and CR4 (CD11c/CD18) to HUVEC was demonstrated by flow cytometry. The presence of the corresponding mRNAs was confirmed by reverse transcriptase-polymerase chain reaction (RT-PCR) and sequencing of the amplified cDNA fragments. When HUVEC were treated with inflammatory mediators, chemotactic agents or the secretagogue phorbol-12-myristate-13-acetate (PMA), no change in reactivity to CR1 (CD35) or CR4 (CD11c/CD18) monoclonal antibodies was detected on the surface of the cells compared with untreated cells. The presence of CR1 (CD35) and CR4 (CD11c/CD18) on HUVEC indicates that endothelial cells (EC) have the potential to bind C3b and iC3b, respectively, which both mediate biological effects in the course of complement activation.

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Authors and Affiliations

  1. Ullevål Hospital, University of Oslo, Kirkeveien 166, 0407, Oslo, Norway

    Harald Langeggen (Research Forum) & Egil Johnson

  2. Department of Pediatric Surgery, Ullevål Hospital, University of Oslo, Kirkeveien 166, 0407, Oslo, Norway

    Harald Langeggen (Research Forum)

  3. Institute of Medical Genetics, Ullevål Hospital, University of Oslo, Kirkeveien 166, 0407, Oslo, Norway

    Knut Erik Berge

  4. Department of Gastroenterological Surgery, Ullevål Hospital, University of Oslo, Kirkeveien 166, 0407, Oslo, Norway

    Egil Johnson

  5. Department of Environmental Medicine, National Institute of Public Health, Oslo, Norway

    Geir Hetland

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  3. Egil Johnson
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  4. Geir Hetland
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Langeggen, H., Berge, K.E., Johnson, E. et al. Human Umbilical Vein Endothelial Cells Express Complement Receptor 1 (CD35) and Complement Receptor 4 (CD11c/CD18) in vitro . Inflammation 26, 103–110 (2002). https://doi.org/10.1023/A:1015585530204

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