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Probable interaction between S100A7 and E-FABP in the cytosol of human keratinocytes from psoriatic scales

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Abstract

The overexpression of E-FABP and S100A7 in lesional psoriatic skin suggests a possible link with this hyperproliferative skin disease. In order to investigate a role for the proteins in this disease, the purifications for both proteins were re-analyzed. Moreover, a specific antiserum directed against purified human S100A7 was generated. By SDS-PAGE immunoblotting we show that E-FABP and S100A7 are expressed in cultured human differentiating keratinocytes and confirm their overexpression in psoriatic scales. Gel filtration and non-denaturing PAGE revealed that S100A7 co-purified with E-FABP, indicating an association between the two proteins. Ion-exchange chromatography resulted in the dissociation of the complex. Finally, immunoprecipitations using antiserum against E-FABP revealed that S100A7 co-immunoprecipitated with E-FABP from protein extracts of psoriatic scales. These data indicate that E-FABP and S100A7 might form a complex in the cytosol of human keratinocytes.

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References

  1. Holbrook K: Ultrastructure of the epidermis. In: E.B. Lane, F.M. Watt (eds). The Keratinocyte Handbook. Cambridge University Press, Cambridge, 1994, pp 3–42

    Google Scholar 

  2. Elias PM, Menon GK: Structural and biochemical correlates of the epidermal permeability barrier. Adv Lipid Res 24: 1–26, 1991

    Google Scholar 

  3. Rawlings AV, Scott IR, Harding CR, Browser PA: Stratum corneum moisturization at the molecular level. J Invest Dermatol 103: 731–740, 1994

    Google Scholar 

  4. Siegenthaler G, Hotz R, Chatellard-Gruaz D, Jaconi S, Saurat J-H: Characterization and expression of a novel human fatty acid-binding protein: The epidermal type (E-FABP). Biochem Biophys Res Commun 190: 482–487, 1993

    Google Scholar 

  5. Siegenthaler G, Hotz R, Chatellard-Gruaz D, Didierjean L, Hellman U, Saurat J-H: Purification and characterization of the human epidermal fatty acid-binding protein: Localization during epidermal differentiation in vivo and in vitro. Biochem J 302: 363–371, 1994

    Google Scholar 

  6. Madsen P, Rasmussen HH, Leffers H, Honoré B, Celis LE: Molecular cloning and expression of a novel keratinocyte protein [psoriasis-associated fatty acid binding protein (PA-FABP)] that is highly up-regulated in psoriatic skin and that shares similarity to fatty acid-binding proteins. J Invest Dermatol 99: 299–305, 1992

    Google Scholar 

  7. Siegenthaler G, Roulin K, Chatellard-Gruaz D, Hotz R, Saurat L-H, Hellman U, Hagens G: A heterocomplex formed by the calciumbinding proteins MRP8 (S100A8) and MRP14 (S100A9) binds unsaturated fatty acids. J Biol Chem 272: 9371–9377, 1997

    Google Scholar 

  8. Masouyé I, Hagens G, van Kuppevelt TH, Madsen P, Saurat JH, Veerkamp JH, Pepper MS, Siegenthaler G: Endothelial cells of the human microvasculature express epidermal fatty acid-binding protein (E-FABP). Circ Res 81: 297–303, 1997

    Google Scholar 

  9. Kragballe K, Voorhees JJ: Eicosanoids in psoriasis-15–HETE on the stage. Dermatologica 174: 209–213, 1987

    Google Scholar 

  10. Fogh K, Herlin T, Kragballe K: Eicosanoids in acute and chronic psoriatic lesions: Leukotriene B4, but not 12–hydroxy-eicosatetraenoic acid, is present in biologically active amounts in acute guttate lesions. J Invest Dermatol 92: 837–841, 1989

    Google Scholar 

  11. Duell EA, Ellis CN, Voorhees LL: Determination of 5,12, and 15–lipoxygenase products in keratomed biopsies of normal and psoriatic skin. J Invest Dermatol 91: 446–450, 1988

    Google Scholar 

  12. Masouyé L, Saurat J-H, Siegenthaler G: Epidermal fatty acid-binding protein in psoriasis, basal and squamous cell carcinomas: An immunohistological study. Dermatology 192: 208–213, 1996

    Google Scholar 

  13. Madsen R, Rasmussen HH, Leffers H, Honoré B, Dejgaard K, Olsen E, KiiI J, Walbum E, Andersen AH, Basse B, Lauridsen JB, Ratz GP, Celis A, Vanderkerckhove L, Celis JE: Molecular cloning, occurrence, and expression of a novel partially secreted protein ‘psoriasin’ that is highly up-regulated in psoriatic skin. J Invest Dermatol 97: 701–712, 1991

    Google Scholar 

  14. Bürgisser D, Siegenthaler G, Kuster T, Hellman U, Hunziker R, Birchler N, Heizmann CW: Amino acid sequence analysis of human S100A7 (Psoriasin) by tandem mass spectrometry. Biochem Biophys Res Com 217: 257–263, 1995

    Google Scholar 

  15. Hoffmann HJ, Oisen E, Etzerodt M, Madsen R, Thogersen HC, Kruse T, Celis LE: Psoriasin binds calcium and is upregulated by calcium to levels that resemble those observed in normal skin. J Invest Dermatol 103: 370–375, 1994

    Google Scholar 

  16. Kligman D, Hilt DC: The S100 protein family. Trends Biochem Sci 13: 437–443, 1988

    Google Scholar 

  17. Rheinwald JH, Green H: Epidermal growth factor and the multiplication of cultured epidermal keratinocytes. Nature 265: 421–424, 1977

    Google Scholar 

  18. Laemmli UK: Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 277: 680–685, 1979

    Google Scholar 

  19. Hagens G, Roulin K, Hotz R, Saurat J-H, Siegenthaler G: The MRP8/14 complex and not the epidermal fatty acid-binding protein is responsible for the translocation of arachidonic acid in human keratinocytes. In: L.Y. Vanderhoek (ed). Frontiers in Bioactive Lipids. Plenum Press, New York, 1996, pp 61–66

    Google Scholar 

  20. Hardas BD, Zhao X, Zhang J, Longqing X, Stoll S, Elder M: Assignment of psoriasin to the chromosomal band 1q12: Coordinate overexpression of clustered genes in psoriasis. J Invest Dermatol 106: 753–758, 1996

    Google Scholar 

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Hagens, G., Roulin, K., Hotz, R. et al. Probable interaction between S100A7 and E-FABP in the cytosol of human keratinocytes from psoriatic scales. Mol Cell Biochem 192, 123–128 (1999). https://doi.org/10.1023/A:1006894909694

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  • DOI: https://doi.org/10.1023/A:1006894909694

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