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Developmental potential of pig embryos reconstructed by use of sow versus pre-pubertal gilt oocytes after somatic cell nuclear transfer*

Published online by Cambridge University Press:  18 January 2013

Juan Li ,
Hanne Skovsgaard Pedersen ,
Rong Li ,
Janne Adamsen ,
Ying Liu ,
Mette Schmidt ,
Stig Purup  and
Henrik Callesen
Juan Li
Affiliation:
College of Animal Science and Technology, Nanjing Agricultural University, 210095, Jiangsu Province, Nanjing, Wei Gang 1, China. Department of Animal Science, Aarhus University, Blichers Alle 20, DK-8830 Tjele, Denmark.
Hanne Skovsgaard Pedersen
Affiliation:
Department of Animal Science, Aarhus University, Blichers Alle 20, DK-8830 Tjele, Denmark.
Rong Li
Affiliation:
Department of Animal Science, Aarhus University, Blichers Alle 20, DK-8830 Tjele, Denmark.
Janne Adamsen
Affiliation:
Department of Animal Science, Aarhus University, Blichers Alle 20, DK-8830 Tjele, Denmark.
Ying Liu
Affiliation:
Department of Animal Science, Aarhus University, Blichers Alle 20, DK-8830 Tjele, Denmark.
Mette Schmidt
Affiliation:
Veterinary Reproduction and Obstetrics, Faculty of Health and Medical Sciences, University of Copenhagen, DK-1870 Frederiksberg C, Denmark.
Stig Purup
Affiliation:
Department of Animal Science, Aarhus University, Blichers Alle 20, DK-8830 Tjele, Denmark.
Henrik Callesen*
Affiliation:
Department of Animal Science, Aarhus University, Blichers Alle 20, DK-8830 Tjele, Denmark.
*
All correspondence to: Henrik Callesen. Department of Animal Science, Aarhus University, Blichers Alle 20, DK-8830 Tjele, Denmark. Tel: +45 8715 7989. Fax: +45 8715 4249. e-mail: Henrik.Callesen@agrsci.dk
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Summary

In this study, the developmental ability of cloned embryos using gilt versus sow oocytes was evaluated under the hypothesis that the efficiency of nuclear transfer using gilt oocytes was lower than that of sow oocytes, but that it could be optimized. Five experiments were performed with routine production of cloned embryos with sow oocytes serving as the control. Results showed that: Experiment 1: Blastocyst rates of cloned embryos with gilt oocytes was about half compared with control. Experiment 2: An extended maturation time of 48 h used for gilt oocytes resulted in lower blastocyst rates after cloning. Experiment 3: Development of cloned embryos with gilt oocytes was improved by co-culture with sow oocytes. Experiment 4: After maturation of gilt oocytes using follicular fluid from gilt instead of sow, the oocytes were sorted into large and small oocytes, and after cloning, blastocyst rates were higher using large gilt oocytes compared with small oocytes; however, the rate remained lower compared with control. Experiment 5: Six sow recipients received a total of 503 morulae and blastocysts cloned from gilt oocytes (four recipients) and 190 cloned from sow oocytes (two recipients). All recipients became pregnant and went to term, resulting in 26 (gilt oocytes) and six (sow oocytes) piglets. In conclusion, results confirmed that nuclear transfer efficiency was higher using sow versus gilt oocytes, but the use of gilt oocytes can be optimized by sorting after ooplasm size following maturation and by maturing gilt and sow oocytes together.

Keywords

Co-cultureCytoplasmHand-made cloningIn vitroPiglets
Type
Research Article
Information
Zygote , Volume 22 , Issue 3 , August 2014 , pp. 356 - 365
Copyright
Copyright © Cambridge University Press 2013 

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Footnotes

*

This work was carried out at the Department of Animal Science, Aarhus University, Denmark.

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