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Cellular and metabolic damage induced by desiccation in recalcitrant Araucaria angustifolia embryos

Published online by Cambridge University Press:  19 September 2008

L. Salmen Espindola ,
M. Noin ,
F. Corbineau  and
D. Côme
L. Salmen Espindola
Affiliation:
Universite Pierre et Marie Curie, Laboratoire de Physiologie Vegetale Appliquee, Tour 53, 1er etage, 4 place Jussieu, 75252 Paris cedex 05, France
M. Noin
Affiliation:
Universite Pierre et Marie Curie, Laboratoire de Cytologie Experimentale et Morphogenèse Vegetale, Bat N2, 4 place Jussieu, 75252 Paris cedex 05, France
F. Corbineau
Affiliation:
Universite Pierre et Marie Curie, Laboratoire de Physiologie Vegetale Appliquee, Tour 53, 1er etage, 4 place Jussieu, 75252 Paris cedex 05, France
D. Côme*
Affiliation:
Universite Pierre et Marie Curie, Laboratoire de Physiologie Vegetale Appliquee, Tour 53, 1er etage, 4 place Jussieu, 75252 Paris cedex 05, France
*
* Correspondence
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Abstract

Embryos of Araucaria angustifolia seeds showed no dormancy; they germinated easily at temperatures ranging from 10° to 30°C, and the thermal optimum was about 25–30°C; they were recalcitrant. At harvest, their mean moisture content (dry weight basis) was about 120% and they completely lost viability when their moisture content fell to about 30%. The cotyledons were more sensitive to dehydration than the radicle. Dehydration induced deterioration of cell membranes as indicated by a high increase in leakage of solutes. It also resulted in damage in the nuclei, which was not repaired upon rehydration. During desiccation, respiratory activity decreased; however, O2 uptake was not an indication of germination ability, since it was significantly affected only when embryo moisture content reached the critical value of 30%. The decrease in the capacity to convert 1-aminocyclopropane 1-carboxylic acid to ethylene, which was observed at 30–60 min of dehydration, was a very early indicator of deterioration in embryos. Desiccation resulted also in a rapid decrease in the ability for protein synthesis as measured by [35S]methionine incorporation into total protein; 50% inhibition was observed after 30–60 min of desiccation for both axis and cotyledons.

Keywords

recalcitrant seedgerminationAraucaria angustifoliadesiccation sensitivityrespiratory activityethyleneprotein synthesis
Type
Short Communication
Information
Seed Science Research , Volume 4 , Issue 2 , June 1994 , pp. 193 - 201
Copyright
Copyright © Cambridge University Press 1994

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References

Akalehiywot, T. and Bewley, J.D. (1980) Desiccation of oat grains during and following germination, and its effects upon protein synthesis. Canadian Journal of Botany 58, 2349–2355.CrossRefGoogle Scholar
Becwar, M.K., Stanwood, P.S. and Roos, E.E. (1982) Dehydration effects on imbibitional leakage from desiccation sensitive seeds. Plant Physiology 69, 1132–1135.CrossRefGoogle ScholarPubMed
Berjak, P., Farrant, J.M. and Pammenter, N.W. (1989) The basis of recalcitrant seed behaviour. Cell biology of the homoiohydrous seed condition. pp 89–108 in Taylorson, R.B. (Ed.) Recent advances in the development and germination of seeds. New York, Plenum Press.CrossRefGoogle Scholar
Berjak, P., Pammenter, N.W. and Vertucci, C. (1992) Homoiohydrous (recalcitrant) seeds: developmental status, desiccation sensitivity and the state of water in axes of Landolphia kirkii Dyer. Planta 186, 249–261.CrossRefGoogle ScholarPubMed
Bewley, J.D. (1979) Physiological aspects of desiccation tolerance. Annual Review of Plant Physiology 30, 195–238.CrossRefGoogle Scholar
Chin, H.F. and Roberts, E.H. (1980) Recalcitrant crop seeds. Kuala Lumpur, Tropical Press SDN, DHDI.Google Scholar
Côme, D. and Corbineau, F. (1992) Les semences et le froid. pp 401–461 in Côme, D. (Ed.) Les végétaux et le froid. Paris, Hermann.Google Scholar
Corbineau, F. and Côme, D. (1986) Experiments on the storage of seeds and seedlings of Symphonia globulifera L. f. (Guttiferae). Seed Science and Technology 14, 585–591.Google Scholar
Corbineau, F. and Côme, D. (1988) Storage of recalcitrant seeds of four tropical species. Seed Science and Technology 16, 97–103.Google Scholar
Corbineau, F. and Côme, D. (1989) Germination and storage of recalcitrant seeds of some tropical forest tree species. Annales des Sciences Forestières 46, 89s–91s.CrossRefGoogle Scholar
Dickie, J.B., May, K., Morris, S.V.A. and Titley, S.E. (1991) The effects of desiccation on seed survival in Acer platanoides L. and Acer pseudoplatanus L. Seed Science Research 1, 149–162.CrossRefGoogle Scholar
Ellis, R.H., Hong, T.D. and Roberts, E.H. (1991) An intermediate category of seed storage behaviour? II. Effects of provenance, immaturity, and imbibition on desiccation-tolerance in coffee. Journal of Experimental Botany 42, 653–657.CrossRefGoogle Scholar
Farrant, J.M., Pammenter, N.W. and Berjak, P. (1985) The effect of drying rate on viability retention of recalcitrant propagules of Avicennia marina. South African Journal of Botany 51, 432–438.CrossRefGoogle Scholar
Farrant, J.M., Pammenter, N.W. and Berjak, P. (1988) Recalcitrance – a current assessment. Seed Science and Technology 16, 155–166.Google Scholar
Farrant, J.M., Pammenter, N.W. and Berjak, P. (1989) Germination associated events and the desiccation sensitivity of recalcitrant seeds – a study on three unrelated species. Planta 176, 189–198.CrossRefGoogle Scholar
Farrant, J.M., Pammenter, N.W. and Berjak, P. (1993) Seed development in relation to desiccation tolerance: a comparison between desiccation-sensitive (recalcitrant) seeds of Avicennia marina and desiccation-tolerant types. Seed Science Research 3, 1–13.CrossRefGoogle Scholar
Finch-Savage, W.E. (1992) Seed development in the recalcitrant species Quercus robur L.: germinability and desiccation tolerance. Seed Science Research 2, 17–22.CrossRefGoogle Scholar
Fu, J.R., Zhang, B.Z., Wang, X.F., Qiao, Y.Z. and Huang, X.L. (1990) Physiological studies on desiccation, wet storage and cryopreservation of recalcitrant seeds of three fruit species and their excised embryonic axes. Seed Science and Technology 18, 743–754.Google Scholar
Fu, J.R., Xia, Q.H. and Tang, L.F. (1993) Effects of desiccation on excised embryonic axes of three recalcitrant seeds and studies on cryopreservation. Seed Science and Technology 21, 85–95.Google Scholar
Givelberg, A., Horowitz, M. and Poljakoff-Mayber, A. (1984) Solute leakage from Solanum nigrum L. seeds exposed to high temperatures during imbibition. Journal of Experimental Botany 35, 1754–1763.CrossRefGoogle Scholar
Hendricks, T.W. and Taylorson, R.B. (1976) Variation in germination and amino acid leakage of seeds with temperature related to membrane phase change. Plant Physiology 58, 7–11.CrossRefGoogle ScholarPubMed
Hong, T.D. and Ellis, R.H. (1990) A comparison of maturation drying, germination, and desiccation tolerance between developing seeds of Acer pseudo-platanus L. and Acer platanoides L. New Phytologist 116, 589–596.CrossRefGoogle Scholar
Hong, T.D. and Ellis, R.H. (1992) Development of desiccation tolerance in Norway maple (Acer platanoides L.) seeds during maturation drying. Seed Science Research 2, 169–172.CrossRefGoogle Scholar
Hueck, K. (1953) Distribuição e habitat natural do pinheiro do Paraná. Boletim Faculdade Filosofia Ciências Letras, Botanica 10, 5–24.CrossRefGoogle Scholar
King, M.W. and Roberts, E.H. (1979) The storage of recalcitrant seeds. Achievements and possible approaches. Rome, International Board for Plant Genetic Resources.Google Scholar
Magini, E. (1962) Forest seed handling, equipment and procedures. II. Seed treatment, storage, testing and transport. Unasylva 16, 20–35.Google Scholar
McKersie, B.D. and Stinson, R.H. (1980) Effects of dehydration on leakage and membrane structure in Lotus corniculatus L. seeds. Plant Physiology 66, 316–320.CrossRefGoogle ScholarPubMed
Misra, S. and Bewley, J.D. (1986) Desiccation of Phaseolus vulgaris seeds during and following germination, and its effect upon the translatable mRNA population of the seed axes. Journal of Experimental Botany 37, 364–374.CrossRefGoogle Scholar
Moore, R.P. (1973) Tetrazolium staining for assessing seed quality. pp 347–366 in Heydecker, W. (Ed.) Seed ecology. London, Butterworths.Google Scholar
Odawara, S.A., Watanabe, H. and Imaseki, H. (1977) Involvement of cellular membrane in regulation of ethylene production. Plant Physiology 18, 569–575.Google Scholar
Olsen, D.F. Jr., and Gabriel, W.J. (1974) Acer L. maple. pp 187–194 in Schopmeyer, C.S. (Tech. Co-ord.) Seeds of woody plants in the United States. Agriculture Handbook No. 450. Washington DC, Forest Service, USDA.Google Scholar
Porter, A.J.R., Borlakoglu, J.T. and John, P. (1986) Activity of the ethylene-forming enzyme in relation to plant cell structure and organization. Journal of Plant Physiology 125, 207–216.CrossRefGoogle Scholar
Priestley, D.A. (1986) Seed aging. Implications for seed storage and persistence in the soil. Ithaca, New York, Cornell University Press.Google Scholar
Pritchard, H.W. and Prendergast, F.G. (1986) Effects of desiccation and cryopreservation on the in vitro viability of embryos of the recalcitrant seed species Araucaria hunsteinii K. Schum. Journal of Experimental Botany 182, 1388–1397.CrossRefGoogle Scholar
Roberts, E.H. (1973) Predicting the storage life of seeds. Seed Science and Technology 1, 499–514.Google Scholar
Roberts, E.H., King, M.W. and Ellis, R.H. (1984) Recalcitrant seeds: their recognition and storage. pp 38–52 in Holden, J.H. and Williams, J.T. (Eds) Crop genetic resources: conservation and evaluation. London, George Allen & Unwin.Google Scholar
Tompsett, P.B. (1984) Desiccation studies in relation to the storage of Araucaria seed. Annals of Applied Biology 105, 581–586.CrossRefGoogle Scholar
Tompsett, P.B. (1987) Desiccation and storage studies on Dipterocarpus seeds. Annals of Applied Biology 110, 371–379.CrossRefGoogle Scholar
Tompsett, P.B. and Pritchard, H.W. (1993) Water status changes during development in relation to the germination and desiccation tolerance of Aesculus hippocastanum seeds. Annals of Botany 71, 107–116.CrossRefGoogle Scholar