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Multilocus sequence typing and clonal population genetic structure of Cyclospora cayetanensis in humans

Published online by Cambridge University Press:  12 July 2017

JUNQIANG LI ,
YANKAI CHANG ,
KE SHI ,
RONGJUN WANG ,
KANDA FU ,
SHAN LI ,
JINLING XU ,
LITING JIA ,
ZHENXIN GUO  and
LONGXIAN ZHANG Open the ORCID record for LONGXIAN ZHANG [Opens in a new window]
JUNQIANG LI
Affiliation:
College of Animal Science and Veterinary Medicine, Henan Agricultural University, Zhengzhou 450002, China International Joint Research Laboratory for Zoonotic Diseases of Henan, Zhengzhou 450002, China
YANKAI CHANG
Affiliation:
College of Animal Science and Veterinary Medicine, Henan Agricultural University, Zhengzhou 450002, China International Joint Research Laboratory for Zoonotic Diseases of Henan, Zhengzhou 450002, China
KE SHI
Affiliation:
College of Animal Science and Veterinary Medicine, Henan Agricultural University, Zhengzhou 450002, China International Joint Research Laboratory for Zoonotic Diseases of Henan, Zhengzhou 450002, China
RONGJUN WANG
Affiliation:
College of Animal Science and Veterinary Medicine, Henan Agricultural University, Zhengzhou 450002, China International Joint Research Laboratory for Zoonotic Diseases of Henan, Zhengzhou 450002, China
KANDA FU
Affiliation:
Huaihe Hospital of Henan University, Kaifeng 475000, China
SHAN LI
Affiliation:
The PLA No. 155 Central Hospital, Kaifeng 475000, China
JINLING XU
Affiliation:
Henan Province People's Hospital, Zhengzhou 450003, China
LITING JIA
Affiliation:
The Third Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, China
ZHENXIN GUO
Affiliation:
Zhengzhou Children's Hospital, Zhengzhou 450018, China
LONGXIAN ZHANG*
Affiliation:
College of Animal Science and Veterinary Medicine, Henan Agricultural University, Zhengzhou 450002, China International Joint Research Laboratory for Zoonotic Diseases of Henan, Zhengzhou 450002, China
*
*Corresponding author: College of Animal Science and Veterinary Medicine, Henan Agricultural University, 95 Wenhua Road, Zhengzhou 450002, P. R. China. E-mail: zhanglx8999@henau.edu.cn; zhanglx8999@gmail.com
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Summary

To investigate the prevalence of Cyclospora cayetanensis in a longitudinal study and to conduct a population genetic analysis, fecal specimens from 6579 patients were collected during the cyclosporiasis – prevalent seasons in two urban areas of central China in 2011–2015. The overall incidence of C. cayetanensis infection was 1·2% (76/6579): 1·6% (50/3173) in Zhengzhou and 0·8% (26/3406) in Kaifeng (P < 0·05), with infections in all age groups (P > 0·05). All the isolates clustered in the C. cayetanensis clade based on the small subunit ribosomal RNA gene sequence phylogenetic analysis. There were 45 specimens positive for all the five C. cayetanensis microsatellite loci, and formed 29 multilocus genotypes (MLGs). The phylogenetic relationships of 54 distinct MLGs (including 25 known reference MLGs), based on the concatenated multilocus sequences, formed three main clusters. A population structure analysis showed that the 79 isolates (including 34 known reference isolates) of C. cayetanensis produced three distinct subpopulations based on allelic profile data. In conclusion, we determined the frequency of C. cayetanensis infection in humans in Henan Province. The clonal population structure of the human C. cayetanensis isolates showed linkage disequilibrium and three distinct subpopulations.

Keywords

Cyclospora cayetanensprevalencelongitudinalmultilocusclonal population
Type
Research Article
Information
Parasitology , Volume 144 , Issue 14 , December 2017 , pp. 1890 - 1897
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Copyright
Copyright © Cambridge University Press 2017 

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