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Changes in adult Nippostrongylus brasiliensis during the development of immunity to this nematode in rats

1. Changes in ultrastructure

Published online by Cambridge University Press:  06 April 2009

D. L. Lee
D. L. Lee
Affiliation:
Houghton Poultry Research Station, Houghton, Huntingdon, England
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Extract

Ultrastructural studies on adult Nippostrongylus brasiliensis removed at varying stages after infection from rats given a single dose of 2000 infective larvae have shown that changes in their cytology occur and that these can be correlated with the onset and development of immunity to this nematode in the host. The most noticeable changes are resorption of spermatozoa by the male reproductive tract, a drop in egg production, changes in the cytology of the intestinal cells, and the appearance of many large droplets, which look like droplets of lipid, in the cytoplasm of the hypodermis, muscles, excretory glands, reproductive system and the intestine. There are no obvious changes in the structure of the cuticle of the nematode during the course of infection. Possible reasons for the onset of these changes in the nematode are discussed.

Much of this work was carried out during the tenure of grants from the U.S. Public Health Service (grant number AI 04275) and from the Agricultural Research Council while I was working at the Molteno Institute in Cambridge. I wish to thank the late Professor J. D. Boyd and Dr C. C. D. Shute of the Department of Anatomy, Cambridge, for permission to use the electron microscopes, Mrs B. Fisher for technical assistance and Mr Paul Rogers for assistance with the photography.

Type
Research Article
Information
Parasitology , Volume 59 , Issue 1 , February 1969 , pp. 29 - 39
Copyright
Copyright © Cambridge University Press 1969

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References

REFERENCES

Brambell, M. R. (1965). The distribution of a primary infestation of Nippostrongylus brasiliensis in the small intestine of laboratory rats. Parasitology 55, 313–24.Google Scholar
Chandler, A. C. (1937). Studies on the nature of immunity to intestinal helminths. Am. J. Hyg. 26, 309–21.Google Scholar
Favard, P. (1961). Evolution des ultrastructures cellulaires au cours de le spermatogenèse de l'Ascaris (Ascaris megalocephala, Schrank = Parascaris equorum, Goerze). Annls Sci. nat. (Zool.), Ser. 12e, 3, 53–152.Google Scholar
Gordon, A. B., Miller, L. R. & Bensch, K. G. (1963). Fixation of tissue culture cells for ultrastructural cytochemistry. Expl Cell Res. 31, 440–43.Google Scholar
Jamuar, M. P. (1966 a). Studies of spermiogenesis in a nematode, Nippostrongylus brasiliensis. J. Cell Biol. 31, 381–96.Google Scholar
Jamuar, M. P. (1966 b). Cytochemical and electron microscope studies on the pharynx and intestinal epithelium of Nippostrongylus brasiliensis. J. Parasit. 52, 1116–28.CrossRefGoogle ScholarPubMed
Keeling, J. E. D. (1960). The effects of ultra-violet radiation on Nippostrongylus muris. I. Irradiation of infective larvae: lethal and sublethal effects. Ann. trop. Med. Parasit. 54, 182–91.Google Scholar
Lee, D. L. (1960). The distribution of glycogen and fat in Thelastoma bulhõesi (Magalhães, 1900), a nematode parasitic in cockroaches. Parasitology 50, 247–59.CrossRefGoogle ScholarPubMed
Lee, D. L. (1965). The cuticle of adult Nippostrongylus brasiliensis. Parasitology 55, 173–81.Google Scholar
Ogilvie, B. M. & Hockley, D. J.Effects of immunity on Nippostrongylus brasiliensis adult worms: reversible and irreversible changes in infectivity, reproduction and morphology. J. Parasit. (in the Press).Google Scholar
Roberts, L. S. & Fairbairn, D. (1965). Metabolic studies on adult Nippostrongylus brasiliensis (Nematoda: Trichostrongyloidea). J. Parasit. 51, 129–38.CrossRefGoogle ScholarPubMed
Rosenbluth, J. (1965). Ultrastructural organisation of obliquely striated muscle fibres in Ascaris lumbricoides. J. Cell Biol. 25, 495–515.Google Scholar
Sarles, M. P. & Taliaferro, W. H. (1936). The local points of defense and the passive transfer of acquired immunity to Nippostrongylus muris in rats. J. infect Dis. 59, 207–20.Google Scholar
Taliaferro, W. H. (1940). The mechanism of acquired immunity to infections with parasitic worms. Physiol. Rev. 20, 469–92.CrossRefGoogle Scholar
Thorsen, R. E. (1953). Studies on the mechanism of immunity in the rat to the nematode Nippostrongylus muris. Am J. Hyg. 58, 1–15.Google Scholar