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Development and ultrastructure of Trachipleistophora hominis n.g., n.sp. after in vitro isolation from an AIDS patient and inoculation into athymic mice

Published online by Cambridge University Press:  06 April 2009

W. S. Hollister ,
E. U. Canning ,
E. Weidner ,
A. S. Field ,
J. Kench  and
D. J. Marriott
W. S. Hollister
Affiliation:
Department of Biology, Imperial College of Science, Technology and Medicine, London SW7 2AZ, UK
E. U. Canning
Affiliation:
Department of Biology, Imperial College of Science, Technology and Medicine, London SW7 2AZ, UK
E. Weidner
Affiliation:
Department of Zoology and Physiology, Louisiana State University, Baton Rouge, Louisiana 70803–1725, USA
A. S. Field
Affiliation:
St Vincent's Hospital, Darlinghurst, Sydney, NSW 2010, Australia
J. Kench
Affiliation:
St Vincent's Hospital, Darlinghurst, Sydney, NSW 2010, Australia
D. J. Marriott
Affiliation:
St Vincent's Hospital, Darlinghurst, Sydney, NSW 2010, Australia
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Summary

Continuous culture was achieved in several cell lines of a microsporidium obtained from the skeletal muscle of an AIDS patient. Development in COS-1 and RK13 cells was prolific. Spores from the original biopsy were also inoculated into athymic mice by i.m. and i.p. routes. Infection was found in several organs as well as in skeletal muscle after a few weeks. All stages were surrounded by an electron-dense surface coat. Meronts had 2–4 nuclei and divided by binary fission. In sporogony the surface coat became separated from the plasma membrane to form a sporophorous vesicle, within which division into sporoblasts was effected by repeated binary fissions. The number of sporoblasts (and later spores) within the sporophorous vesicles varied from 2 to > 32 and the sizes of the vesicles varied, according to the number of spores contained therein, from 5 μm diameter to 14·0 × 11·0 μm. Spores measured 4·0 × 2·4 μm and had a prominent posterior vacuole. The parasite differs from the genus Pleistophora in that it does not form multinucleate sporogonial plasmodia and that the sporophorous vesicle enlarges during sporogony and its wall is not a multilayered structure. It is proposed to place it in a new genus and species Trachipleistophora hominis n.g., n.sp.

Keywords

Trachipleistophora hominis n.g., n.sp.skeletal muscleAIDS patientin vitro cultureathymic miceexperimental infection.
Type
Research Article
Information
Parasitology , Volume 112 , Issue 1 , January 1996 , pp. 143 - 154
Copyright
Copyright © Cambridge University Press 1996

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References

REFERENCES

Cali, A. (1991). General microsporidian features and recent findings on AIDS isolates. Journal of Protozoology 38, 625–30.Google ScholarPubMed
Cali, A. & Owen, R. L. (1988). Microsporidiosis. In Laboratory Diagnosis of Infectious Diseases: Principles and Practice (ed. Balows, A., Hausler, W. J. & Lennette, E. H.), pp. 928–49 New York: Springer-Verlag.Google Scholar
Canning, E. U. & Hazard, E. I. (1982). Genus Pleistophora Gurley, 1893: an assemblage of at least three genera. Journal of Protozoology 29, 39–49.Google Scholar
Canning, E. U., Hazard, E. I. & Nicholas, J. P. (1979). Light and electron-microscopy of Pleistophora sp. from skeletal muscle of Blennius pholis. Protistologica 15, 317–32.Google Scholar
Canning, E. U., Killick-Kendrick, R. & Killick-Kendrick, M. (1991). A new microsporidian parasite, Flabelliforma montana n.g., n.sp. infecting Phlebotomus ariasi (Diptera, Psychodidae) in France. Journal of Invertebrate Pathology 5, 71–81.CrossRefGoogle Scholar
Canning, E. U. & Lom, J. (1986). The Microsporidia of Vertebrates, London: Academic Press.Google Scholar
Canning, E. U. & Nicholas, J. P. (1980). Genus Pleistophora (Phylum Microspora): redescription of the type species, Pleistophora typicalis Gurley, 1893 and ultrastructural characterization of the genus. Journal of Fish Diseases 3, 317–38.Google Scholar
Chupp, G. L., Alroy, J., Adelman, L. S., Breen, J. C. & Skolnik, P. R. (1992). Myositis due to Pleistophora (Microsporidia) in a patient with AIDS. Clinical Infectious Diseases 16, 15–21.CrossRefGoogle Scholar
Didier, E. S., Didier, P. J., Friedberg, D. N., Stenson, S. M., Orenstein, J. M., Yee, R. W., Tio, F. O., Davis, R. M., Vossbrinck, C., Millichamp, N. & Shadduck, J. (1991). Isolation and characterization of a new human microsporidian, Encephalitozoon hellem (n.sp.) from three AIDS patients with keratoconjunctivitis. Journal of Infectious Diseases 163, 617–21.Google Scholar
Gurley, R. (1893). Classification of the Myxosporidia, a group of protozoan parasites infesting fishes. Bulletin of the United States Fisheries Commission for 1891 11, 407–20.Google Scholar
Ledford, D. K.,Overman, M. D., Gonzalvo, A., Cali, A., Mester, S. W. & Lockey, R. F. (1985). Microsporidiosis myositis in a patient with acquired immunodeficiency syndrome. Annals of Internal Medicine 102, 628–30.Google Scholar
Lom, J. & Corliss, J. O. (1967). Ultrastructural observations on the development of the microsporidian protozoon Plistophora hyphessobryconis Schaperclaus. Journal of Protozoology 14, 141–52.Google Scholar
McDougall, R. J., Tandy, M. W., Boreham, R. E., Stenzel, D. J. & O'Donoghue, P. J. (1993). Incidental finding of a microsporidian parasite from an AIDS patient. Journal of Clinical Microbiology 31, 436–9.Google Scholar
Schwartz, D. A., Bryan, R. T., Hewan-Lowe, K. O., Visvesvara, G. S., Weber, R., Cali, A. & Angritt, P. (1992). Disseminated microsporidiosis (Encephalitozoon hellem) and acquired immunodeficiency syndrome. Archives of Pathology and Laboratory Medicine 116, 660–8.Google Scholar
Sprague, V., Becnel, J. J. & Hazard, E. I. (1992). Taxonomy of Phylum Microspora. Critical Reviews in Microbiology 18, 285–95.Google Scholar
Vávra, J., Dahbiová, R., Hollister, W. S. & Canning, E. U. (1993). Staining of microsporidian spores with optical brighteners, with recommendations for the diagnosis of AIDS-associated human microsporidiosis. Folia Parasitologica 40, 267–72.Google Scholar