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Phylogenetic position of Sphincterodiplostomum Dubois, 1936 (Digenea: Diplostomoidea) with description of a second species from Pantanal, Brazil

Published online by Cambridge University Press:  11 February 2021

T.J. Achatz Open the ORCID record for T.J. Achatz [Opens in a new window] ,
J.A. Bell Open the ORCID record for J.A. Bell [Opens in a new window] ,
F.T.V. Melo Open the ORCID record for F.T.V. Melo [Opens in a new window] ,
A. Fecchio Open the ORCID record for A. Fecchio [Opens in a new window]  and
V.V. Tkach Open the ORCID record for V.V. Tkach [Opens in a new window]
T.J. Achatz
Affiliation:
Department of Biology, University of North Dakota, Starcher Hall, 10 Cornell Street Stop 9019, Grand Forks, ND58202, USA
J.A. Bell
Affiliation:
Department of Biology, University of North Dakota, Starcher Hall, 10 Cornell Street Stop 9019, Grand Forks, ND58202, USA
F.T.V. Melo
Affiliation:
Laboratory of Cell Biology and Helminthology ‘Prof. Dr Reinalda Marisa Lanfredi’, Institute of Biological Sciences, Federal University of Pará, Belém, PA, Brazil
A. Fecchio
Affiliation:
Programa de Pós-Graduação em Ecologia e Conservação da Biodiversidade, Universidade Federal de Mato Grosso, Cuiabá, MT, Brazil
V.V. Tkach*
Affiliation:
Department of Biology, University of North Dakota, Starcher Hall, 10 Cornell Street Stop 9019, Grand Forks, ND58202, USA
*
Author for correspondence: V.V. Tkach, E-mail: vasyl.tkach@und.edu
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Abstract

Sphincterodiplostomum is a monotypic genus of diplostomid digeneans that parasitize fish-eating birds in the neotropics. The type species Sphincterodiplostomum musculosum has a unique, dorsal, tubular invagination in the opisthosoma with a muscular sphincter. Whereas larvae of S. musculosum are relatively commonly reported in Neotropical fish helminth surveys, adult specimens from birds are rarely collected. Prior to our study, no DNA sequence data for S. musculosum were available. Our molecular and morphological study of mature and immature adult Sphincterodiplostomum specimens from three species of birds and one species of crocodilian revealed the presence of at least two species of Sphincterodiplostomum in the neotropics. We provide the first molecular phylogeny of the Diplostomoidea that includes Sphincterodiplostomum. In addition, this is the first record of S. musculosum from caimans, along with the first record of fully mature adult S. musculosum from green kingfisher Chloroceryle americana. The new species of Sphincterodiplostomum (Sphincterodiplostomum joaopinhoi n. sp.) can be morphologically distinguished from S. musculosum based on the anterior extent of vitelline follicles, narrower prosoma, substantially smaller holdfast organ and structure of tegumental spines. Our data revealed 0.7% interspecific divergence in 28S and 10.6–11.7% divergence in cox1 sequences between the two Sphincterodiplostomum species.

Keywords

DiplostomidaeSphincterodiplostomum musculosumSphincterodiplostomum joaopinhoi n. spmolecular phylogenyPantanal
Type
Research Paper
Information
Journal of Helminthology , Volume 95 , 2021 , e6
Copyright
Copyright © The Author(s), 2021. Published by Cambridge University Press

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References

Achatz, TJ, Curran, SS, Patitucci, KF, Fecchio, A and Tkach, VV (2019a) Phylogenetic affinities of Uvulifer spp. (Digenea: Diplostomidae) in the Americas with description of two new species from Peruvian Amazon. Journal of Parasitology 105, 704–717.CrossRefGoogle Scholar
Achatz, TJ, Dmytrieva, I, Kuzmin, Y and Tkach, VV (2019b) Phylogenetic position of Codonocephalus (Digenea, Diplostomoidea), an unusual diplostomid with progenetic metacercariae. Journal of Parasitology 105, 821–826.CrossRefGoogle Scholar
Achatz, TJ, Pulis, EE, Fecchio, A, Schlosser, IJ and Tkach, VV (2019c) Phylogenetic relationships, expanded diversity and distribution of Crassiphiala (Digenea, Diplostomidae), agents of black spot disease in fish. Parasitology Research 118, 2781–2787.CrossRefGoogle Scholar
Achatz, TJ, Pulis, EE, Junker, K, Binh, TT, Snyder, SD and Tkach, VV (2019d) Molecular phylogeny of the Cyathocotylidae (Digenea, Diplostomoidea) necessitates systematic changes and reveals a history of host and environment switches. Zoologica Scripta 48, 545–556.CrossRefGoogle Scholar
Achatz, TJ, Pulis, EE, González-Acuña, D and Tkach, VV (2020) Phylogenetic relationships of Cardiocephaloides spp. (Digenea, Diplostomoidea) and the genetic characterization of Cardiocephaloides physalis from Magellanic Penguin, Spheniscus magellanicus, in Chile. Acta Parasitologica 65, 525–534.CrossRefGoogle ScholarPubMed
Blasco-Costa, I and Locke, SA (2017) Life history, systematics and evolution of the Diplostomoidea Poirier, 1886: progress, promises and challenges emerging from molecular studies. Advances in Parasitology 98, 167–225.CrossRefGoogle ScholarPubMed
Delgado, EA, Tantaleán, VM, Martínez, RR and Mondragón, MA (2017) Trematodos en Hoplerythrinus unitaeniatus (Erythrinidae) «Shuyo» y Pterodoras granulosus (Doradidae) «Cahuara» del Distrito de Yurimaguas, Provincia Alto Amazonas, Loreto, Perú. Revista De Investigaciones Veterinarias Del Perú 28, 461–467.CrossRefGoogle Scholar
Dubois, G (1936) Nouveaux principes de classification des Trématodes du groupe des Strigeida (Notes préliminaires). Revue suisse de zoologie 43, 507–515.CrossRefGoogle Scholar
Dubois, G (1938) Monographie des Strigeida (Trematoda). Mémoires de la Société Neuchâteloise des Sciences Naturelles 6, 1–535.Google Scholar
Hernández-Mena, DI, García-Varela, M and Pérez-Ponce de León, G (2017) Filling the gaps in the classification of the Digenea Carus, 1863: systematic position of the Proterodiplostomidae Dubois, 1936 within the superfamily Diplostomoidea Poirier, 1886, inferred from nuclear and mitochondrial DNA sequences. Systematic parasitology 94, 833–848.CrossRefGoogle ScholarPubMed
Kumar, S, Stecher, G and Tamura, K (2016) MEGA7: molecular evolutionary genetics analysis version 7.0 for bigger datasets. Molecular Biology and Evolution 33, 1870–1874.CrossRefGoogle ScholarPubMed
Locke, SA, Van Dam, AR, Caffara, M, Pinto, HA, López-Hernández, D and Blanar, CA (2018) Validity of the Diplostomoidea and Diplostomida (Digenea, Platyhelminthes) upheld in phylogenomic analysis. International Journal for Parasitology 48, 1043–1059.CrossRefGoogle ScholarPubMed
López-Jiménez, A, Pérez-Ponce de León, G and & García-Varela, M (2018) Molecular data reveal high diversity of Uvulifer (Trematoda: Diplostomidae) in Middle America, with the description of a new species. Journal of Helminthology 92, 725–739.CrossRefGoogle ScholarPubMed
Lunaschi, LI and Drago, FB (2006) First report of adult specimens of Sphincterodiplostomum musculosum (Digenea, Diplostomidae). Parasitology International 55, 7–10.CrossRefGoogle Scholar
Lutz, HL, Tkach, VV and Weckstein, JD (2017) Methods for specimen-based studies of avian symbionts. pp. 127–183 in Webster, M (Ed) The role of collections in ornithology: the extended specimen. Studies in avian biology. Boca Raton, CRC Press.Google Scholar
Niewiadomska, K (2002) Family Diplostomidae Poirier, 1886. pp. 167–196 in Gibson, DI, Jones, A and Bray, RA (Eds) Keys to the Trematoda, Vol. 1. Wallingford, CAB International and The Natural History Museum.CrossRefGoogle Scholar
Pérez-Ponce de León, G and Hernández-Mena, DI (2019) Testing the higher-level phylogenetic classification of Digenea (Platyhelminthes, Trematoda) based on nuclear DNA sequences before entering the age of the ‘next-generation’ Tree of Life. Journal of Helminthology 93, 260–276.CrossRefGoogle ScholarPubMed
Queiroz, M, López-Hernández, D, Locke, S, Pinto, H and Anjos, L (2020) Metacercariae of Heterodiplostomum lanceolatum (Trematoda: Proterodiplostomidae) found in Leptodactylus podicipinus (Anura: Leptodactylidae) from Brazil: a morphological, molecular and ecological study. Journal of Helminthology 94, E66.CrossRefGoogle Scholar
Rocha, RS, Pelegrini, LS, Camargo, AA, Abdallah, VD and Azevedo, RK (2015) Sphincterodiplostomum musculosum (Digenea, Diplostomidae) in Geophagus brasiliensis (Perciformes, Cichlidae) collected in a lake at Dois Córregos, São Paulo, Brazil. Ciência Rural 45, 2223–2228.CrossRefGoogle Scholar
Ronquist, F and Huelsenbeck, JP (2003) MRBAYES 3: Bayesian phylogenetic inference under mixed models. Bioinformatics 19, 1572–1574.CrossRefGoogle ScholarPubMed
Szidat, L (1969) Structure, development, and behavior of new strigeatoid metacercariae from subtropical fishes of South America. Journal of the Fisheries Research Board of Canada 26, 753–786.CrossRefGoogle Scholar
Tkach, VV and Pawlowski, J (1999) A new method of DNA extraction from the ethanol-fixed Parasitic worms. Acta Parasitologica 44, 147–148.Google Scholar
Tkach, VV, Achatz, TJ, Pulis, EE, Junker, K, Snyder, SD, Bell, JA, Halajian, A and Melo, FTV (2020) Phylogeny and systematics of the Proterodiplostomidae Dubois, 1936 (Digenea: Diplostomoidea) reflect the complex evolutionary history of the ancient digenean group. Systematic Parasitology 97, 409–439.CrossRefGoogle ScholarPubMed
Zago, AC, Franceschini, L, Ramos, IP, Zica, EOP, Wunderlich, AC, Carvalho, ED and Silva, RJ (2013) Sphincterodiplostomum musculosum (Digenea, Diplostomidae) infecting Steindachnerina insculpta (Characiformes, Curimatidae) in the Chavantes Reservoir, Southeastern Brazil. Revista Brasileira de Parasitologia Veterinária 22, 98–103.CrossRefGoogle ScholarPubMed