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Molecular characterization and expression analysis of soluble trehalase gene in Aphis glycines, a migratory pest of soybean

Published online by Cambridge University Press:  28 February 2013

Raman Bansal ,
M. A. Rouf Mian ,
Omprakash Mittapalli  and
Andy P. Michel
Raman Bansal
Affiliation:
Department of Entomology, Ohio Agricultural Research and Development Center, The Ohio State University, 1680 Madison Avenue, Wooster, OH 44691, U.S.A.
M. A. Rouf Mian
Affiliation:
USDA-ARS and Department of Horticulture and Crop Sciences, The Ohio State University, Wooster, OH 44691, U.S.A.
Omprakash Mittapalli
Affiliation:
Department of Entomology, Ohio Agricultural Research and Development Center, The Ohio State University, 1680 Madison Avenue, Wooster, OH 44691, U.S.A.
Andy P. Michel*
Affiliation:
Department of Entomology, Ohio Agricultural Research and Development Center, The Ohio State University, 1680 Madison Avenue, Wooster, OH 44691, U.S.A.
*
*Author for correspondence Phone: +1 330 263 3730 Fax: +1 330 263 3686 E-mail: michel.70@osu.edu
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Abstract

In insects, the enzyme trehalase plays a crucial role in energy metabolism, chitin synthesis and possibly during plant–insect interactions. We have characterized a soluble trehalase gene (Tre-1) from cDNA of Aphis glycines, a serious migratory pest of soybean. The full-length cDNA of Tre-1 in A. glycines (AyTre-1) was 2550 bp long with an open reading frame of 1770 bp that encoded for a 589 amino acid residues protein. Sequence assessment and phylogenetic analysis of the putative protein suggested that the selected cDNA belongs to soluble trehalase group. Quantitative PCR (qPCR) analysis in different tissues and developmental stages revealed peak mRNA levels of AyTre-1 in the gut (compared with other tissues assayed) and highest expression in the second instar compared with the other developmental stages assayed. Interestingly, a significantly increased expression of AyTre-1 (1.9-fold, P < 0.05) was observed in the alate morphs compared with that in apterate morphs. However, there was no significant difference in AyTre-1 expression in A. glycines-nymphs fed with resistant and susceptible plants. Expression patterns identified in this study provide a platform to investigate the role of AyTre-1 in physiological activities such as flight and feeding in A. glycines. The characterization of soluble trehalase gene may help to develop novel strategies to manage A. glycines using trehalase inhibitors and using RNA interference for knock-down of AyTre-1 expression.

Keywords

Aphis glycinessoybean aphidtrehalaseqPCRmigrationalate morphs
Type
Research Paper
Information
Bulletin of Entomological Research , Volume 103 , Issue 3 , June 2013 , pp. 286 - 295
Copyright
Copyright © Cambridge University Press 2013

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