Asian Journal of Surgery

Asian Journal of Surgery

Volume 33, Issue 4, October 2010, Pages 173-180
Asian Journal of Surgery

Original Article
Effects of Ganoderma lucidum on Obstructive Jaundice-induced Oxidative Stress

https://doi.org/10.1016/S1015-9584(11)60003-1Get rights and content
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Objective

Obstructive jaundice develops after occlusion of the common bile duct. Direct hyperbilirubinaemia, which occurs secondary to the condition, causes various life-threatening pathologies. Cytoprotective effects of Ganoderma lucidum (GL) have previously been shown. In this study, the effects of GL on oxidative stress and oxidant DNA damage in experimental obstructive jaundice were evaluated.

Methods

Sixty Wistar albino adult female rats were randomly divided into six weight-matched equal groups: sham group, bile duct ligated group (BDL); after sham operation 250 mg/kg/d of GL administered group, after sham operation 500 mg/kg/d of GL administered group, after bile duct ligation 250 mg/kg/d of GL administered (GL1BDL) group, and after bile duct ligation 500 mg/kg/d of GL administered (GL2BDL) group. GL polysaccharide was orally administered to the rats via gavage tube once a day for 14 days after bile duct ligation.

Results

The plasma malondialdehyde levels of the GL1BDL and GL2BDL groups were significantly lower than those of the BDL group (p < 0.01). The plasma 8-hydroxy-2′-deoxyguanosine levels of the GL1BDL and GL2BDL groups were significantly lower than those of the BDL group (p < 0.001). The liver tissue Cu-Zn superoxide dismutase level of the GL2BDL group was significantly higher than that of the BDL group (p < 0.05).

Conclusion

GL protected against DNA and liver tissue damage by reducing oxidative stress in obstructive jaundice.

Key Words

direct hyperbilirubinaemia
DNA damage
experimental cholestasis
Ganoderma lucidum
obstructive jaundice
oxidative stress

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