Elsevier

Neuroscience Letters

Volume 323, Issue 3, 3 May 2002, Pages 195-198
Neuroscience Letters

Daily rhythm of glutathione peroxidase activity, lipid peroxidation and glutathione levels in tissues of pinealectomized rats

https://doi.org/10.1016/S0304-3940(02)00144-1Get rights and content

Abstract

Melatonin is a component of the antioxidant defense system since it has radical scavenging and antioxidant activities. In the present study, we aimed to investigate the endogenous rhythm of antioxidant enzyme glutathione peroxidase (GSH-Px) activity, oxidized glutathione (GSSG) and lipid peroxidation levels in tissues of pinealectomized rats (PINX). Rats were sacrificed by decapitation at 4 h intervals. GSH-Px activity, GSSG and lipid peroxidation levels showed a daily rhythm both in controls and in PINX rats. GSH-Px and GSSG exhibited the peak levels after the peak time of melatonin which was determined previously by other groups. Lipid peroxidation levels increased progressively during the night and started to decline before the GSH-Px peak time. These findings suggest that endogenous melatonin is involved in the night time increase of GSH-Px activity and GSSG levels and modulates the daily rhythm pattern of GSH-Px. In conclusion, pinealectomy which eliminates the melatonin rhythm has a supressor effect on GSH-Px activity levels.

References (20)

There are more references available in the full text version of this article.

Cited by (129)

  • An intracerebroventricular injection of amyloid-beta peptide (1–42) aggregates modifies daily temporal organization of clock factors expression, protein carbonyls and antioxidant enzymes in the rat hippocampus

    2021, Brain Research
    Citation Excerpt :

    Interestingly, we found that an intracerebroventricular injection of Aß aggregates modifies daily oscillation of protein carbonyls levels and daily rhythms of clock-related genes and has a differential effect on the oscillating CAT and GPx expression and activity. We and others have observed daily rhythmicity of protein oxidation levels and antioxidant enzymes activity in several mammals’ tissues (Baydas et al., 2002; Coto-Montes et al., 2001; Fonzo et al., 2009; Jiménez-Ortega et al., 2010). Recently, we showed rhythms of protein oxidation in the prefrontal cortex of rat during the light phase of the day (Ledezma et al., 2020).

  • Immune-toxicity effects of scorpion venom on the hypothalamic pituitary adrenal axis during rest and activity phases in a rodent model

    2020, Comparative Biochemistry and Physiology Part - C: Toxicology and Pharmacology
    Citation Excerpt :

    The obtained results at the hypothalamic and cerebral tissues level revealed a day-night variation in antioxidant systems response after the night envenomation with high GSH levels and catalase activity in envenomed mice during the middle of the activity-phase when compared to those of rest-phase. Increased darkness levels of GSH and catalase activity correlate with previous reports (Baydas et al., 2002; Díaz-Muñoz et al., 1985; Sani et al., 2015). However, the pro-oxidants markers (nitrite and hydrogen peroxide) are reduced in all tissues during the activity-phase.

  • Effect of endurance training on diurnal rhythms of superoxide dismutase activity, glutathione and lipid peroxidation in plasma of pinealectomized rats

    2020, Neuroscience Letters
    Citation Excerpt :

    Lipid peroxidation and the activity of antioxidant enzymes SOD and GSH-Px were higher during the dark period and low during the light period but with significantly diminished amplitudes compared to controls in different tissues in rats with pinealectomy [13] suggesting that the endogenous melatonin is involved in the regulatory mechanism of antioxidant enzymes. Furthermore, nocturnal light can suppress both the release of melatonin and the antioxidant signaling system [12]. Exercise has a chronotropic activity and can influence the mammalian circadian clock [17].

View all citing articles on Scopus
View full text