Biochemical and Biophysical Research Communications
Lactoferrin reduces in vitro osteoclast differentiation and resorbing activity
Section snippets
Materials and methods
Reagents. Bovine lactoferrin (bLF) was generously provided by DMV International, Veghel, The Netherlands. Soluble recombinant human RANKL was obtained from Alexis (Alexis, Switzerland). rh-M-CSF, PBS, α-MEM, and heat-inactivated fetal calf serum (FCS) were obtained from Sigma (Fluka, Buchs, Switzerland). Plastic dishes were obtained from Corning (Milan SA, Switzerland).
Rabbit pit assay. Unfractionated bone cells were prepared from long bones from 10-day-old New Zealand rabbits (Chavornay,
Effect of bLF on in vitro bone resorption in rabbit pit assays
We have tested the effect of bLF in a rabbit pit assay system. Rabbit bone cells were cultured for 72 h in the presence of several bLF concentrations (10 μg/ml to 1000 μg/ml). Osteoclastic bone resorbing activity was significantly and dose-dependently reduced. A 50% inhibition of bone resorption was obtained with 200 μg/ml (Fig. 1).
Generation of osteoclast-like cells from human CD14 selected cells
When human CD14 selected cells were cultured on bone slices in the presence of RANKL (25 ng/ml) and M-CSF (30 ng/ml) for 8, 14, and 21 days, multinucleated calcitonin
Discussion
To our knowledge, we have shown for the first time that bovine lactoferrin decreases (i) osteoclast activity in rabbit mixed bone cell cultures (pit assay system) and (ii) osteoclast differentiation of human CD14-selected cells towards osteoclast commitment.
Among the models available to study osteoclast biology, the rabbit pit assay is useful to assess osteoclast activity but not the osteoclast differentiation process. The mixed bone cell population consists of authentic osteoclasts in an
Acknowledgements
We are grateful to Dr. H. Green, Dr. P. Guesry (Nestle Research Center) for critical reading of the manuscript, and Dr. B.O. Oyajobi (University of Texas Health Science Center at San Antonio) for helpful discussions.
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