Maspin overexpression correlates with positive response to primary chemotherapy in ovarian cancer patients☆
Section snippets
Patients and tissue samples
168 ovarian specimens were obtained during surgeries from patients who were treated in the Gynecology Oncology Department, Medical University of Gdańsk (from 04.2004 to 07.2007). The collection of tissues was supervised by a pathologist. Tissue samples were immediately frozen in liquid nitrogen and maintained at − 70 °C. Specimens consisted of 32 normal tissues (obtained from patients who underwent surgery for other gynecologic diseases than ovarian tumors), 42 benign tumors, 10 borderline (LMP)
Western blot analysis of maspin expression in ovarian tissue
Maspin was expressed as a protein band with a mass of 42 kDa. Interestingly, a second band of approximately 35 kDa was also reactive with the maspin antibodies in most tested samples. (The band of 60 kDa was a result of unspecific reaction with secondary antibodies) (Fig. 1). Maspin (42 kDa isoform) was expressed at detectable levels in 17/32 healthy tissue samples (53.1%) with relative expression ranging from 0.03 to 0.7, in 33/42 benign tumors (78.6%, 0.02–2.3), 9/10 borderline tumors (90%,
Discussion
The clinical importance of maspin in human cancers has been investigated since its discovery in 1994 [7]. Experimental studies revealed that maspin suppresses tumor growth, angiogenesis, invasion and metastasis [18], [19], [20], [23], [24]. Maspin is also involved in the process of cell apoptosis [21], [22]. The exact function of maspin as a tumor suppressor is not known, moreover its localization in different cell compartments (cytosol, nucleus, extracellular matrix), suggests that it may be
Conflict of interest statement
The authors declare that there are no conflicts of interest.
Acknowledgments
This work was supported by the Polish Ministry of Science and Higher Education (Grant No. N 40306631/3077).
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Presented in part at the International Congress “Ovarian Cancer” Krakow, June 18–21, 2008.