Elsevier

Vaccine

Volume 28, Issue 40, 14 September 2010, Pages 6627-6636
Vaccine

A protein-based smallpox vaccine protects non-human primates from a lethal monkeypox virus challenge

https://doi.org/10.1016/j.vaccine.2010.07.030Get rights and content

Abstract

Concerns about infections caused by orthopoxviruses, such as variola and monkeypox viruses, drive ongoing efforts to develop novel smallpox vaccines that are both effective and safe to use in diverse populations. A subunit smallpox vaccine comprising vaccinia virus membrane proteins A33, B5, L1, A27 and aluminum hydroxide (alum) ± CpG was administered to non-human primates, which were subsequently challenged with a lethal intravenous dose of monkeypox virus. Alum adjuvanted vaccines provided only partial protection but the addition of CpG provided full protection that was associated with a more homogeneous antibody response and stronger IgG1 responses. These results indicate that it is feasible to develop a highly effective subunit vaccine against orthopoxvirus infections as a safer alternative to live vaccinia virus vaccination.

Keywords

Smallpox
Monkeypox
Vaccinia virus
Subunit vaccine
CpG
Aluminum hydroxide
Baculovirus expressed proteins

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1

These authors contributed equally.

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