Effect of ultrasound treatments on functional properties and structure of millet protein concentrate
Introduction
Proso millet (Panicum miliaceum L.), a comparatively short-season crop, requires little water and is able to grow at a wide range of altitudes. This cereal has considered in food production because of its advantages including high yield, affluence and low cost.[1]. In addition, proso millet has higher (13.4%) protein content than many common cereals such as wheat (10.5%) and rice (6.8–7.4%) [2], [3], [4]. It contains considerable quantity of essential amino acids especially the Sulphur containing amino acids (methionine and cysteine). On the other hand, millets because of their agricultural advantages, health benefits and nutritive values have been received specific alterations as a good food source from developing countries. Health benefits such as, decreasing tumor incidence, reducing blood pressure, cholesterol absorption, preventing cardiovascular diseases and cancer, also nutritive values including provide a variety of nutrients and antioxidants needed for human health, have been reported for millets [5], [6]. They are a gluten free cereal and thus is appropriate for people with wheat/gluten allergies [7].
Proteins play different roles in food matrix that named functional properties. According to Kinsella [8], the functional properties are “those physical and chemical properties that influence the behavior of proteins in food systems during processing, storage, cooking and consumption” and which are affected by multiple factors such as pH, drying, heating, ionic strength, storage conditions, presence of reducing agents, and physical, chemical or enzymatic modifications [8].
Among the different physical methods for proteins modifications, ultrasound, which is defined as sound waves having frequency that exceeds the hearing limit of the human ear (∼20 kHz), has simple, cost-effective, energy saving and environment friendly advantages [9], [10]. Generally, ultrasound power is affected by pressure, temperature, intensity, energy and velocity, that based on frequency range it can be divided into high and low energy ultrasound. Frequency between 20 and 100 kHz with high intensity (10–1000 W/cm2) used in high energy ultrasound which caused alterations in mechanical, physical, or chemical/biochemical attributes of proteins because of creation of high pressure (1000 atm) and temperature (5000 K) during cavitation phenomenon. In contrast, frequency between 5 and 10 MHz with low intensity (1 W/cm2) has non-destructive effects and is used to ensure high quality and safety of foods applications [10], [11], [12]. Generation of high power ultrasound could be done with sonication bath and/or transportable cheap ultrasonic immersion probes due to various goals in food manufacturing [13].
In recent years, many researchers have investigated the impact of ultrasound on functional properties of vegetable and animal protein sources specially soy proteins. In this case, the researchers showed strong effect of ultrasound treatment on foaming, solubility, emulsifying and other functional properties of these proteins [11], [14], [15], [16], [17], [18], [19].
Several studies showed that emulsifying performance of egg white protein (20 kHz, 4.27 W, for 20 min) and dairy proteins (20 kHz, 34 W/cm2, for 2 min) improved after high power ultrasound treatment [17], [20]. In a study conducted on animal and vegetable proteins, it was found out that solubility of pea protein concentrate and emulsifying performance of bovine gelatin, egg white protein and pea protein concentrate improved after ultrasound treatment (20 kHz, acoustic intensity of ∼34 W/cm2 for 2 min) [17]. In another study, different ultrasound power (200 W, 400 W, 600 W) were used to modify emulsifying properties of Soy protein isolates (SPI). They found increase in emulsifying properties of SPI after using ultrasound. The results showed the middle power ultrasound (400 W) treated protein had a lower saturation surface load and a higher protein adsorption fraction that can explain its better emulsifying capability [15]. Hu et al. [18] studied the effects of 20 kHz (low- frequency) ultrasound at different time (15 or 30 min) and power (200, 400 or 600 W) on soy protein isolate structural and functional properties. They did not find significant change in the protein electrophoretic patterns. The surface hydrophobicity and protein solubility of SPI were increased with increase in both of time and power of ultrasonic treatment [18] that could lead to increase in emulsifying and foaming activity.
Due to the burgeoning world population and on the other hand enhancing cost and confined supply of animal proteins, new sources of plant proteins for use in food applications, will need to be developed [8]. The main novelty of this work is choosing millet protein concentrate as low cost and nutritious protein source. It can be show different behavior from other proteins mentioned in literature in different intensity and time. Therefore, the purpose of the present study was to examine the impact of high power ultrasound (20 kHz) on improvement of emulsifying, foaming and solubility of millet protein concentrates to determine the possibility of using these proteins in different food applications such as emulsifier and egg replacer. In addition, FTIR, DSC, Zeta potential and SDS-Page methods was used to evaluate the relationship between physicochemical and structural properties of native and modified protein.
Section snippets
Materials
Proso millet seeds were purchased from Seed & Plant Improvement Institute, Karaj, Iran. The seeds were cleaned by hand, sieved to remove the foreign materials, and milled using a laboratory-scale hammer miller (laboratory Mill 3100, Perten co.) in the quality control lab of Ard daran Co belonging to Tak Makaron co. Alborz, Iran. The resulting millet flour were packed in polyethylene bags and stored in a refrigerator and used during one week after milling. All chemicals used in this study were
Solubility
As can be seen in Fig. 1, solubility of MPC after ultrasound treatment in all of the used times and amplitudes were significantly higher than the untreated sample with the highest solubility in 73.95 W/cm2 intensity for 12.5 min. The results indicated that increase in US time from 5 to 20 min at 18.4 W/cm2 intensity did not show significant improvement in solubility of MPC. On the other hand, increase in level of US time from 12.5 to 20 min at 73.95 W/cm2 intensity reduced it. In various
Conclusions
In this study, MPC was affected by the high power US probe (20 kHz) in varying intensities and times (18.4, 29.58, and 73.95 W/cm2 for 5, 12.5 and 20 min) and the impact of this process on the functional and structural properties of MPC was investigated.
US treatments significantly (P < .05) increased the solubility of the native MPC (65.8 ± 0.6%) at all sonicated times with maximum solubility that recorded at D2 treatment (96.9 ± 0.82%). FC of MPC was also significantly affected by the US
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