Clinical practiceIsolation and preservation of human isletAnalysis on Donor and Isolation-Related Factors of Successful Isolation of Human Islet of Langerhans From Human Cadaveric Donors
Section snippets
Cadaveric Donors
Seventy-five cadaveric pancreata were procured for islet isolation after obtaining informed consent from the relatives. Six cases were excluded in the analysis due to technical failures during either the digestion or the purification phases of the islet isolation process. Donor factors reviewed in charts included age, gender, body mass index (weight in kg/height in m2), cause of death (trauma vs nontrauma), cold ischemic time (time from cessation of blood flow to pancreas to time of intraductal
Results
Seventy-five pancreata were procured for isolation of human islets of Langerhans after exclusion of six technical failures. Mean age of donors was 31 years, and mean cold ischemia time was 6.9 hours. Quality control assays on isolated islets were mostly acceptable. The mean islet recovery was 216.0 × 103 (IEQ) or 2840 (IEQ) per gram of pancreas. Mean purity was 54%. When islet isolation recovered >100,000 IEQ were arbitrarily considered successful, the success rate of islet isolation was 76%.
Discussion
Experience in islet isolations at several center has demonstrated that many factors affect the ability to recover human islets during organ procurement and isolation processes.2, 3, 4 Our center commenced to isolate islets of Langerhans in 1992 as the first center in Korea. It has facilities and techniques according to FDA/c-GMP requirements. Islet yield definitely increased year by year in our center, which showed the importance of experience.
Donor age is one of the important factors affecting
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This work was supported by grants from the Asan Institute for Life Science (2005-211).