Trends in Plant Science
Technology of the MonthMH-seq for Functional Characterization of Open Chromatin in Plants
Section snippets
ADVANTAGES
MH-seq has simple procedures that are easily adapted to a variety of plant species. It will be a powerful approach for profiling open chromatins in plants.
MH-seq is comparable with DNase-seq and ATAC-seq.
MH-seq enables detection of distinct types of open chromatin associated with active, repressive, or poised chromatin states, thereby providing more comprehensive regulatory information about the genome.
Joint profiling by MH-seq and histone ChIP-seq in one MNase treatment experiment will provide
CHALLENGES
MH-seq needs further improvement when used on limited amounts of plant cells or tissues.
Single-cell-based MH-seq can truly reflect the homogeneity of chromatin states in cells; it will attract broader applications, but it is not easy to establish in plants.
Joint profiling of chromatin accessibility, gene expression, nucleosome occupancy, and histone modifications in the same cells can mitigate the potential bias from integrating separate omics data but is difficult to achieve in plants.
Nucleus
Acknowledgments
The authors thank their funding sources from the National Natural Science Foundation of China (31571579), the Postgraduate Research and Practice Innovation Program of Jiangsu Province (KYCX18_0644), and the Fundamental Research Funds for the Central Universities (KYYJ201808).
References (0)
Cited by (5)
An efficient CRISPR–Cas12a promoter editing system for crop improvement
2023, Nature Plants