Original article
Labeling of nucleosides with fluorescamine and detection by spectrofluorometer for End Stage Renal Disease

https://doi.org/10.1016/j.sjbs.2013.02.005Get rights and content
Under a Creative Commons license
open access

Abstract

Nucleosides are characterized as biomarkers in AIDS, Alzheimer, tumor, breast cancer and various malignant diseases. In the present work a direct method for the detection of nucleosides (adenosine, cytidine, uridine and guanosine) from urine samples has been developed. Nucleosides represent the extent of damage in genetic material, analysis of nucleosides by ultrasonic assisted microextraction effectively eliminates the interfering constituent of urine. This has made it a highly selective and sensitive method to analyze the nucleosides with a lower limit of detection 0.220 μmol/L and Limit of quantitation 0.660 μmol/L. The method has been validated with good linearity and correlation of coefficients of the calibration curves was higher than 0.997. The coefficients were in the range of 0.11–16.92% (inter-day) and 0.38–16.43% (intra-day), respectively.

Keywords

ESRD (end stage renal disease)
Nucleosides (cytidine, uridine, adenosine and guanosine)
Ultrasonic assisted microextraction

Cited by (0)

Peer review under responsibility of King Saud University.

  1. Download : Download full-size image