Vpx mediated degradation of SAMHD1 has only a very limited effect on lentiviral transduction rate in ex vivo cultured HSPCs☆
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Abbreviations
HSPCs
hematopoietic stem and progenitor cells
VLPs
viral like particles
IL-2R
interleukin-2 receptor
SIN
self-inactivating
SAMHD1
sterile alpha motif domain and HD domain-containing protein 1
dGTP
deoxyguanosine-triphosphate
dNTPs
deoxynucleoside triphosphates
dNs
deoxynucleosides
DCs
dendritic cells
PBMCs
peripheral blood mononuclear cells
MDMs
monocyte-derived macrophages
SCF
stem cell factor
Flt3L
Fms-related tyrosine kinase 3 ligand
IL-3
interleukin-3
TPO
thrombopoietin
PEI
polyethylenimine
GAPDH
glyceraldehyde 3-phosphate dehydrogenase
MNE
mean normalized gene expression
EVF
efavirenz
RTV
raltegravir
TGV
total gray values
MFI
mean fluorescence intensity
Keywords
SAMHD1
dNTP pools
Transduction
Hematopoietic stem and progenitor cells (HSPCs)
Gene engineering
Lentiviral vectors
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Author contribution: DL designed, conducted and analyzed all experiments. ES, AA, M-A.R, SI, C-N.K, BK, O-T.K assisted in some experiment. DL, AA and RS wrote the paper.
Copyright © 2015 Published by Elsevier B.V.