Experimental radiobiologyBone marrow transplantation rescues intestinal mucosa after whole body radiation via paracrine mechanisms
Section snippets
Animals
Mice were housed at the animal care facility of the NHRI (Tainan, Taiwan). The facility was approved by the National Association for the Accreditation of Laboratory Animal Care, Taiwan, and was maintained in accordance with the regulations and standards of the NHRI Animal Council’s procedural and ethical guidelines. C57Bl/6 mice, 8–12 weeks old, were purchased from the National Laboratory Animal Center (NLAC).
Radiation, BMT and tissue preparation
WBI of 13 Gy was delivered using a linear accelerator (Philip SL 75-5), at a dose rate
BMCM alone may improve the intestinal survival of mice from radiation damage
According to our previous observations [6], we postulated that cytokines secreted from BM cells, either exogenously transplanted or endogenously from host, contribute to intestinal regeneration after radiation. We used conditioned medium from primary BM cell cultures to demonstrate the paracrine effect of BM cells on the intestine. The amount of each cytokine in the concentrated BM conditioned medium (BMCM) was controlled by ELISA assays (bFGF: 46.61 ± 8.77, IGF:1138.54 ± 15.56, VEGF: 274.93 ± 21.93,
Discussion
The present study demonstrated a mechanism for the engagement of BM cells in the repair of radiation damage to the intestinal mucosa. The physiological response to intestinal injury has suggested that replacement of dead cells might depend either on reentry into the cell cycle of differentiated cells surviving injury or on the contribution of BM-derived or resident stem cells. However, it was a rare event of BM cells homeing in to the intestine and replacing damaged epithelial or stromal cells
Acknowledgments
We thank Dr. Helen H.W. Chen for administrative support and Mr. Hsin-Chun Chang for technical help. This study was supported by Grant NSC 97-2321-B-400-004-MY2 and NSC100-2314-B-400-001 – from National Science Council, Taipei, Taiwan.
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