Construction of a doxycycline inducible adipogenic lentiviral expression system
Under a Creative Commons license
open access
Highlights
► We constructed a lentiviral Tet On overexpression plasmid controlled by aP2 promoter. ► The combination of TRE + rtTAadv in vectors resulted in the lowest basal expression. ► TRE + rtTAadv also gave the best inducibility by Dox in HIB-1B and 3T3-L1 cell lines. ► A truncated aP2 promoter conferred adipogenic expression in HIB-1B cells.
Keywords
Tetracycline inducible expression
Lentiviral plasmid
Adipose-specific promoter
Adipocyte
Cited by (0)
- 1
Inserm, U1016, Institut Cochin, Paris, France
Copyright © 2012 Elsevier Inc.