Molecular NeuroscienceCognitive performance and age-related changes in the hippocampal proteome
Section snippets
Animals and behavioral testing
Fisher 344xBrown Norway (F1) male rats (10 and 27 months of age) were obtained from the NIA colony at Harlan Industries (Indianapolis, IN, USA). Animals were singly housed in polycarbonate cages with food (Purina Mills, Richmond, IN, USA) and water was available ad libitum. The animal rooms were kept at a constant temperature and humidity and maintained on a 12-h light/dark cycle. The animal facilities were fully accredited by the American Association for Accreditation of Laboratory Animal Care
Behavioral results
Animals were subdivided from a cohort of 15 animals (5 Young and 10 Old animals) based on their performance on the training blocks and probe trial phases of the Morris water maze and the absence of gross, peripheral underlying age-related pathology (i.e. tumors, cardiovascular hypertrophy, enlarged kidneys) observed at necropsy. This assessment was necessary since the presence of peripheral disease may influence behavioral results. The absence of significant gross pathology in the experimental
Discussion
Age is a primary risk factor for cognitive decline in rodents, non-human primates and humans, indicated by increasingly impaired performance in tasks of learning and memory. Although the etiology of age-related cognitive decline, and specifically impaired hippocampally-dependent learning and memory, remains unclear, cognitive decline is associated with impaired function of many cellular processes, including, but not limited to, decreased glucose metabolism and blood flow as well as alterations
Acknowledgments
The authors wish to thank the Penn State Mass Spectrometry Facility for technical assistance, Joseph Freeman for editorial assistance, and Tracy Marrow for illustration preparation. This work was supported by R01AG026607 and P01AG11370 (to W.E.S.).
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