In vitro study of improved wound-healing effect of bioactive borate-based glass nano-/micro-fibers

doi:10.1016/j.msec.2015.05.049

Materials Science and Engineering: C

Volume 55, 1 October 2015, Pages 105-117

https://doi.org/10.1016/j.msec.2015.05.049 Get rights and content

Highlights

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Bioactive glass nano-/micro-materials were effectively used for tissue wound healing.
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The wound-healing effects of silicate-based 45S5, borate-based 13-93B3 and 1605 fibers were investigated.
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Glass conversion rates were compared under either static or dynamic-flow modes.
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Glass compositions and flow rates greatly influenced bioactivity and cell migration.
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These results can be potentially used as a guide for bioactive glass material fabrication in the future.

Abstract

Because of the promising wound-healing capability, bioactive glasses have been considered as one of the next generation hard- and soft-tissue regeneration materials. The lack of understanding of the substantial mechanisms, however, indicates the need for further study on cell–glass interactions to better interpret the rehabilitation capability. In the present work, three bioactive glass nano-/micro-fibers, silicate-based 45S5, borate-based 13-93B3 and 1605 (additionally doped with copper oxide and zinc oxide), were firstly compared for their in vitro soaking/conversion rate. The results of elemental monitoring and electron microscopic characterization demonstrated that quicker ion releasing and glass conversion occurred in borate-based fibers than that of silicate-based one. This result was also reflected by the formation speed of hydroxyapatite (HA). This process was further correlated with original boron content and surrounding rheological condition. We showed that an optimal fiber pre-soaking time (or an ideal dynamic flow rate) should exist to stimulate the best cell proliferation and migration ability. Moreover, 13-93B3 and 1605 fibers showed different glass conversion and biocompatibility properties as well, indicating that trace amount variation in composition can also influence fiber's bioactivity. In sum, our in vitro rheological module closely simulated in vivo niche environment and proved a potentially improved wound-healing effect by borate-based glass fibers, and the results shall cast light on future improvement in bioactive glass fabrication.

Graphical abstract

Keywords

Silicate-/borate-based bioactive glass fibers

Glass conversion

Hydroxyapatite formation

Dynamic flow module

Cell proliferation/migration

Soft tissue wound-healing

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Qingbo Yang received his BS degree in bioengineering from Zhengzhou University, MS degree in molecular biology and biochemistry from Shanghai University in China. He joined Dr. Yinfa Ma's group since September, 2010 and he currently is a PhD candidate in analytical chemistry. His research interests include nanomaterial cytotoxicity and bioactivity, single cell and single molecule level imaging and analysis, soft tissue wound-healing effect of bioactive glass nano-fibers. He is currently working as Research Assistant in the Center for Single Particle, Single Cell and Single Molecule Monitoring (CS³M) at Missouri University of Science and Technology.

Sisi Chen is a graduate student in Chemistry Department at Missouri University of Science and Technology. She received her BS degree in school of chemical biology and pharmaceutical sciences at Capital Medical University in 2012 in Beijing, China. She joined Dr. Yinfa Ma's research group in 2012 and her research interests including analytical chemistry and bioactive material characterization and evaluation. She is currently focusing on cancer biomarker analysis using a UPLC–MS/MS system. Effort has also been put into the wound healing mechanism study of different types of bioactive glass nanofibers as well as an NIH funded bio-sensor development project.

Honglan Shi received her PhD in analytical chemistry from Missouri University of Science and Technology in May, 2010. She is an associate research professor in Department of Chemistry at Missouri University of Science and Technology. Her research mainly focus on development of advanced analytical techniques and methods for bioanalytical and environmental applications including state-of-the-art instrument development and manufacturing, bioactive glass–biofluid–bioorganism interaction study by advanced analytical technologies, advanced test kit developments and manufacturing, method development for rapid characterization and quantification of engineered nanomaterials, development of novel economical and green technologies for water treatment, trace emerging pollutants analysis and control drinking water.

Dr. Hai Xiao is the Bell Distinguished Professor of Electrical and Computer Engineering at Clemson University. He received his Ph.D. degree in electrical engineering from Virginia Tech in 2000. He was an associate professor then a professor (2006–2013) with the Department of Electrical and Computer Engineering at Missouri University of Science and Technology. Before that, he was an assistant professor of Electrical Engineering at New Mexico Institute of Mining and Technology. Dr. Xiao's current research interests include photonic and microwave sensors and instrumentation for applications in energy, intelligent infrastructure, clean-environment, biomedical sensing/imaging, and national security.

Yinfa Ma received his PhD in analytical chemistry and minor PhD in biochemistry from Iowa State University. He is currently a Curators' Teaching Professor in Department of Chemistry at Missouri University of Science and Technology, and Associate Dean of College of Arts, Science, and Business. His research focuses on bio-analysis and bio-separations, environmental monitoring, and single molecule and single cell imaging, by using variety of state-of-art instruments, such as high performance liquid chromatography (HPLC), high performance capillary electrophoresis (HPCE), GC–MS, HPLC–MS, HPCE–MS, gel electrophoresis, size-exclusion chromatography, and home-built single molecule and single cell imaging system.

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