Construction of extracellular microenvironment to improve surface endothelialization of NiTi alloy substrate

https://doi.org/10.1016/j.msec.2015.05.047Get rights and content

Highlights

  • Biofunctional multilayer films mimicking extracellular microenvironment were successfully fabricated.

  • Multilayered structure stimulated the biological responses of endothelial cells.

  • The approach affords an efficient approach for surface endothelialization of stent implant.

Abstract

To mimic extracellular microenvironment of endothelial cell, a bioactive multilayered structure of gelatin/chitosan pair, embedding with vascular endothelial growth factor (VEGF), was constructed onto NiTi alloy substrate surface via a layer-by-layer assembly technique. The successful fabrication of the multilayered structure was demonstrated by scanning electron microscopy, atomic force microscopy, contact angle measurement, attenuated total reflection-fourier transform infrared spectroscopy and X-ray photoelectron spectroscopy, respectively. The growth behaviors of endothelial cells on various NiTi alloy substrates were investigated in vitro. Cytoskeleton observation, MTT assay, and wound healing assay proved that the VEGF-embedded multilayer structure positively stimulated adhesion, proliferation and motogenic responses of endothelial cells. More importantly, the present system promoted the nitric oxide production of endothelial cells. The approach affords an alternative to construct extracellular microenvironment for improving surface endothelialization of a cardiovascular implant.

Keywords

Surface modification
Layer-by-layer assembly
Extracellular microenvironment
Endothelialization
Vascular endothelial growth factor

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