Short communicationThe prevalence and genotype diversity of Human Rotavirus A circulating in Thailand, 2011–2014
Section snippets
Introductions
Rotaviruses belong to the Reoviridae family, genus Rotavirus. Based on their antigenic properties, rotaviruses are classified into eight species designated A through H. Rotavirus A (RVA) is commonly found in humans and causes acute watery diarrhea worldwide, especially in children younger than 5 years of age (Parashar et al., 2006). The virus contains 11 segmented double-stranded RNAs, which are translated into six structural (viral proteins [VPs]) and six non-structural proteins (NSP). The
Study population
A total of 688 stool samples were collected over a 3-year period (26 May 2011 to 9 August 2014). Samples were obtained from individuals presented with acute diarrhea defined by three or more abnormally loose or watery stools within 24 h. A total of 157 samples were collected from the Chulalongkorn Memorial Hospital in Bangkok and 531 samples from the Chumphae Hospital in Khon Kaen. Patients included in this study were between the ages of 1 day and 15 years. Samples were initially tested using the
Study population and RVA prevalence
To determine what proportion of diarrhea in young children was attributable to RVA infection, a total of 688 samples were evaluated (Table S1). The vast majority of the samples comprised children ≤ 5 years old. Children aged 1–5 years old (mode 12 months; median 12 months) represented most individuals, in part due to the many samples from Khon Kaen (Fig. 1A). Meanwhile, samples from Bangkok were mostly from patients between 7–12 months (mode 12 months, median 12 months). Overall, there were more males
Acknowledgments
This study was supported by The National Research University Project, Office of the Higher Education Commission (WCU 58-006-HR, WCU007-HR57, WCU 001-HR57), The National Research Council of Thailand, The Research Chair Grant from The National Science and Technology Development Agency (NSTDA), Chulalongkorn University Centenary Academic Development Project (CU56-HR01), the Ratchadaphiseksomphot Endowment Fund of Chulalongkorn University (RES560530093), the Outstanding Professor of Thailand
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Cited by (33)
Pre-vaccine rotavirus surveillance in Buenos Aires, Argentina. Characterization of an emergent G1P[8] strain associated to fatal cases in 2014
2020, Infection, Genetics and EvolutionCitation Excerpt :However, this cannot be asserted based on a single gene, but, unfortunately, we were unable to amplify additional genes from this sample from 2013. The phylogenetic study of the VP7 gene of the five G1P[8] isolates from 2014, and the one from 2013 demonstrated that these Argentine strains are very closely related to a group of Thai, Vietnamese, and Russian human strains and a group of Vietnamese porcine strains circulating since 2012 [Chieochansin et al., 2016; Phan et al., 2016]. This relationship is supported by the sequence study of the other three genes, VP4, VP6, and NSP4.
Whole genome characterization of feline-like G3P[8] reassortant rotavirus A strains bearing the DS-1-like backbone genes detected in Vietnam, 2016
2019, Infection, Genetics and EvolutionCitation Excerpt :The search identified the VP7 gene of RVA/Human-wt/CHN/E2451/2011 and Human-wt/THA/CU-B1263/KK/2011/G3P[9] as 98.8% and 98.5% identical with that of the Vietnamese G3P[8] strains, respectively (Table 1). As both strains were reported as AU-1/FRV-1-like strains (Wang et al., 2013; Chieochansin et al., 2016), a phylogenetic tree was constructed to place the VP7 genes of these strains together with those of Vietnamese G3P[8] strains in the phylogenetic context of the G3 VP7 genes of feline/canine RVA origin including those of feline/canine-like human RVA strains (Fig. 1). In this tree the VP7 genes of the Vietnamese G3P[8] strains clustered together with those of Chinese E2451 and Thai CU-B1263/KK as well as feline FRV-1 and feline Cat 2 strains to form a lineage supported by a high bootstrap value.
Pediatric hospitalizations attributable to rotavirus gastroenteritis among Cambodian children: Seven years of active surveillance, 2010–2016
2018, VaccineCitation Excerpt :Hoa-Tran et al. revealed that the genetic transfer of a bovine G8 VP7 gene, likely from India, to a Taiwanese human G8 sequence was the common ancestor to the strain circulating in Vietnam, a reassortment event estimated to have occurred 2–7 years before its detection. Since each of the analyzed G8P[8] samples shared this genetic reassortment, and due to the geo-temporal proximity of the G8P[8] emergence in Cambodia, Vietnam and Thailand, further evaluation of this reassortant strain and its hypothesized efficiency in human transmission is warranted [16,30]. Remarkable in what is absent from this large Cambodian sample of genotypic data, there was no detection of the G12P[8] genotype.
Complete genome sequence analysis of rare G4P[6] rotavirus strains from human and pig reveals the evidence for interspecies transmission
2018, Infection, Genetics and EvolutionCitation Excerpt :The G4P[6] genotype, one of the RVA strains that could be detected in both human and animal, was first identified in 1975 in neonate from Great Britain and piglet from the US (Estes, 2013; Palombo and Bishop, 1994). In human, G4P[6] RVA strains have been sporadically detected in children with diarrhea from many countries (Bowen et al., 2016; Chieochansin et al., 2016; Hull et al., 2011; Kim et al., 2014; Kirkwood et al., 2010; Mukherjee et al., 2010; Mullick et al., 2014; Razafindratsimandresy et al., 2013). In host species other than human, most G4P[6] RVA strains have been reported in pigs with high prevalence in Asia and Europe (Vlasova et al., 2017).
Clinical and molecular epidemiologic trends reveal the important role of rotavirus in adult infectious gastroenteritis, in Shanghai, China
2017, Infection, Genetics and EvolutionCitation Excerpt :Phylogenetic analysis of the VP7 genes demonstrated that there were six lineages and eleven sublineages in global G9 strains in accordance with an accepted nomenclature (Phan et al., 2007). Thirty-two G9 strains analyzed in the present study were located in sublineage VIe (specific amino acid substitution, H144) of lineage VI together with some other strains reported in Japan, Thailand, Russia, USA, Solomon Islands and Zimbabwe, and primarily, with Chinese strains detected in children from Beijing (BJ-Q94, BJ-CR7611, BJ-CR8537, X1302), Shanghai (SPH0144), Yunnan (ZT-12N-T66) and Zhejiang (WZ810), from 2010 to date (Shen et al., 2013; Yamamoto et al., 2015; Chieochansin et al., 2016; Jones et al., 2016; Li et al., 2016) (Fig. 2A). These G9-VIe strains shared 95.4% to 100% nucleotide (nt) and 95.0% to 100% amino acid (aa) homology, except for strain SH-RV69 (with shared identity of 93.2% nt and 93.9% aa).