Opinion articlesHLA-G production by human IVF embryos: Can it be measured reliably?
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Acknowledgements
The authors are members of “EMBIC”, a European Network of Excellence (http://www.embic.org) within the 6th Framework Programme of the European Union (LSHM-CT-2004-512040). They acknowledge the fruitful collaboration of many clinicians at ART Centres in Oxford, Poissy and Toulouse.
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Noninvasive methods of embryo selection
2018, Reproductomics: The -Omics Revolution and Its Impact on Human Reproductive MedicineThe effect of the biochemical marker soluble human leukocyte antigen G on pregnancy outcome in assisted reproductive technology - A multicenter study
2013, Fertility and SterilityCitation Excerpt :There has been some criticism of the use of the optical density (OD) value as our sHLA-G unit value. It is interesting that no previous group reporting on applying an sHLA-G assay has reached a consensus on the appropriate way to report such results (22–25, 27, 29–33). We would like to report our results as an internationally agreed upon unit, but no such consensus exists.
Cumulative results including obstetrical and neonatal outcome of fresh and frozen-thawed cycles in elective single versus double fresh embryo transfers
2010, Fertility and SterilityCitation Excerpt :Among them, the selection of the best oocytes by quantitative and qualitative analysis of spindle and zona retardance (36–38) and the metabolic profiling of oocytes and embryos (39–44) might be beneficial in the selection of oocytes and embryos with good developmental potential, leading to implantation, conception, and birth of a healthy child. Immunological biomarkers in individual follicular fluids and embryo supernatants should also help to assess the implantation potential of embryos (45, 46). Another approach for optimizing delivery rates after SET is to extend the embryo culture to day 5 and to transfer the embryo at the blastocyst stage (47).
Classical and non-classical Major Histocompatibility Complex class I gene expression in in vitro derived bovine embryos
2009, Journal of Reproductive ImmunologyCitation Excerpt :The identification of a non-invasive marker of embryo developmental potential would allow clinical embryologists to select the best embryos for transfer, leading to higher IVF pregnancy rates and potentially a reduction in high risk multiple pregnancies. However, the issue of the sensitivity of the sHLA-G ELISAs has been raised (Sageshima et al., 2007) and explored in detail by Sargent et al. (2007), with the authors concluding that further collaboration between research groups is required to establish a more standardized and reproducible approach. In contrast to the situation in mice and humans, knowledge of the bovine MHC-I is relatively limited.
Soluble HLA-G in IVF/ICSI embryo culture supernatants does not always predict implantation success: A multicentre study
2009, Reproductive BioMedicine OnlineSoluble human leukocyte antigen-G and pregnancy success
2008, Reproductive BioMedicine Online