Effect of Agitation of EDTA with 808-Nanometer Diode Laser on Removal of Smear Layer

doi:10.1016/j.joen.2013.07.016

Journal of Endodontics

Volume 39, Issue 12, December 2013, Pages 1589-1592

https://doi.org/10.1016/j.joen.2013.07.016 Get rights and content

Abstract

Introduction

Near-infrared diode lasers can be used for several applications, which range from disinfection to smear layer removal in endodontics. This study evaluated the efficacy of agitation of 15% EDTA with an 808-nm diode laser on removal of the smear layer.

Methods

Sixty extracted human maxillary central incisor teeth were instrumented up to ProTaper F4 (Dentsply Maillefer, Ballagues, Switzerland) and then randomly divided into 6 groups (n = 10 for each group) according to the different final irrigating protocols as follows: 5% sodium hypochlorite for 120 seconds performed with the NaviTip (Dentsply Maillefer, Ballaigues, Switzerland) (control group); 15% EDTA for 120 seconds performed with the NaviTip; and agitation of 15% EDTA with an 808-nm diode laser for 10, 20, 30, and 40 seconds. Specimens were observed under a scanning electron microscope, and open dentinal tubules were counted using Adobe Photoshop software (Adobe Systems, San Jose, CA). The data were analyzed with 1-way analysis of variance and Tukey post hoc tests (P = .05).

Results

The number of open dentinal tubules was higher in the middle thirds than in the apical thirds. The differences between the apical and middle thirds were statistically significant (P < .05). Statistically significant differences were also found between the control group and the other groups in both the middle and apical thirds of the root canals (P < .05).

Conclusions

The results indicated that agitation of 15% EDTA with an 808-nm diode laser for 20 seconds was effective in removing the smear layer in the apical thirds of root canals.

Section snippets

Laser System

An 808-nm wavelength gallium-aluminum-arsenide diode laser (Doctor Smile, Lambda Scientifica Srl, Vicenza, Italy) system equipped with a 20-W power source was used. The laser delivery system used in the study was a fiber-optic cable with a 300-μm size at 2 W in the pulsed mode (10 ms on/10 ms off). The actual power of the parallel fiber-optic tip was 285.71 W/cm².

Specimen Preparation

The protocol of this study was approved by the Research Ethics Committee of Izmir Katip Celebi University, Izmir, Turkey. The study

Results

The number of open dentinal tubules in 120 photomicrographs was counted using Adobe Photoshop software. The means and standard deviations of the open dentinal tubules in the middle and apical thirds were 126.5 ± 91.09 and 51.87 ± 58.70, respectively. According to the statistical analysis, the middle thirds contained a higher number of open dentinal tubules than the apical thirds of the root canals (P < .001).

In the middle third of the root canals, the means and standard deviations of the open

Discussion

Effective chemomechanical preparation of the apical region is especially important for successful root canal treatment. Syringe irrigation is a standard procedure for root canal irrigation, but this technique is not efficient in the apical third of the root canal. It is difficult to completely remove the residual smear layer, particularly in the apical third of the root, because the smaller size of the apical third compared with the other thirds impedes the circulation and action of the

Conclusion

The null hypothesis was rejected. Within the limitations of this study, it can be concluded that 20 seconds of agitation of EDTA with an 808-nm diode laser improved the efficacy of EDTA in removing the smear layer.

Acknowledgment

The authors deny any conflicts of interest related to this study.

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Basic ResearchEffect of Agitation of EDTA with 808-Nanometer Diode Laser on Removal of Smear Layer

Abstract

Introduction

Methods

Results

Conclusions

Section snippets

Laser System

Specimen Preparation

Results

Discussion

Conclusion

Acknowledgment

Dent Clin North Am

J Endod

J Endod

J Endod

J Endod

Oral Surg Oral Med Oral Pathol

Oral Surg Oral Med Oral Pathol

J Endod

J Endod

J Endod

J Endod

J Endod

Basic Research
Effect of Agitation of EDTA with 808-Nanometer Diode Laser on Removal of Smear Layer