Process development for mycelial growth and polysaccharide production in Tricholoma matsutake liquid culture

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Abstract

In this study, the effects of agitation and aeration on mycelial growth and exo-polysaccharide production were examined in batch cultures of Tricholoma matsutake. Agitation was varied from 100 to 300 rpm and aeration was varied from 0.5 to 1.5 vvm. Mycelial growth was 21.87 g/l at 150 rpm, and exo-polysaccharide production was 8.79 g/l at 1.5 vvm. When we analyzed the polysaccharide extractions from the cultured mycelium and the culture broth of T. matsutake, 1.4 g of crude polysaccharide was found per 100 g of dried weight in the cultured mycelium, and 1.47 g/l of polysaccharides was found in the culture broth. In addition, the amounts of β-Glucan in the soluble polysaccharide fractions of the cultured mycelium and culture broth were 75.4% and 83.6%, respectively. The cultured mycelium and the culture broth contained a higher amount of β-Glucan than that of the fruiting body.

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Strain and media

The mycelia were isolated from the fruiting body of T. matsutake, the higher basidiomycetes mushroom, which was collected from Uljin Gyeong-Buk, Korea. They were cultured on an agar plate containing T. matsutake medium (TMM) that was supplemented with antibiotics (50 μg/ml of streptomycin and 60 μg/ml of ampicillin). The TMM agar contained 20 g/l glucose, 1.5 g/l soytone and 1.5 g/l yeast extract. The basal medium for mycelial growth was DMK, which contained 40.0 g/l dextrin, 6.0 g/l yeast

Effect of culture pH

In order to investigate the effect of culture pH on mycelial growth and EPS production, T. matsutake was cultivated in solutions of different acidity (uncontrolled pH and pH of 6) in a 5-l bioreactor. In the case of cultivation without pH control, pH did not change significantly during cultivation. Residual glucose was slowly consumed and remained 17.89 g/l at the 14th day of culture for the T. matsutake liquid culture at uncontrolled pH. Mycelial growth of T. matsutake increased continuously,

Acknowledgments

This study was supported by Technology Development Program for Agriculture and Forestry, Ministry of Agriculture and Forestry, Republic of Korea.

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