Process development for mycelial growth and polysaccharide production in Tricholoma matsutake liquid culture
Section snippets
Strain and media
The mycelia were isolated from the fruiting body of T. matsutake, the higher basidiomycetes mushroom, which was collected from Uljin Gyeong-Buk, Korea. They were cultured on an agar plate containing T. matsutake medium (TMM) that was supplemented with antibiotics (50 μg/ml of streptomycin and 60 μg/ml of ampicillin). The TMM agar contained 20 g/l glucose, 1.5 g/l soytone and 1.5 g/l yeast extract. The basal medium for mycelial growth was DMK, which contained 40.0 g/l dextrin, 6.0 g/l yeast
Effect of culture pH
In order to investigate the effect of culture pH on mycelial growth and EPS production, T. matsutake was cultivated in solutions of different acidity (uncontrolled pH and pH of 6) in a 5-l bioreactor. In the case of cultivation without pH control, pH did not change significantly during cultivation. Residual glucose was slowly consumed and remained 17.89 g/l at the 14th day of culture for the T. matsutake liquid culture at uncontrolled pH. Mycelial growth of T. matsutake increased continuously,
Acknowledgments
This study was supported by Technology Development Program for Agriculture and Forestry, Ministry of Agriculture and Forestry, Republic of Korea.
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