iScience
Volume 23, Issue 10, 23 October 2020, 101547
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Article
Conformational Dynamics of Nonenveloped Circovirus Capsid to the Host Cell Receptor

https://doi.org/10.1016/j.isci.2020.101547Get rights and content
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Highlights

  • Circovirus capsid samples have spontaneous and reversible conformations

  • Receptor binding regulates capsid dynamics

  • Capsid encountering with receptor induces the exposure of the receptor-binding site

  • Capsid interaction with the neutralizing antibody can return to the original conformation

Summary

Circovirus, comprising one capsid protein, is the smallest nonenveloped virus and induces lymphopenia. Circovirus can be used to explore the cell adhesion mechanism of nonenveloped viruses. We developed a single-molecule fluorescence resonance energy transfer (smFRET) assay to directly visualize the capsid's conformational feature. The capsid underwent reversible dynamic transformation between three conformations. The cell surface receptor heparan sulfate (HS) altered the dynamic equilibrium of the capsid to the high-FRET state, revealing the HS-binding region. Neutralizing antibodies restricted capsid transition to a low-FRET state, masking the HS-binding domain. The lack of positively charged amino acids in the HS-binding site reduced cell surface affinity and attenuated virus infectivity via conformational changes. These intrinsic characteristics of the capsid suggested that conformational dynamics is critical for the structural changes occurring upon cell surface receptor binding, supporting a dynamics-based mechanism of receptor binding.

Subject Areas

Molecular Biology
Virology

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5

These authors contributed equally

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