Elsevier

Optik

Volume 180, February 2019, Pages 236-239
Optik

Original research article
Fluorescence detection of boscalid pesticide residues in grape juice

https://doi.org/10.1016/j.ijleo.2018.11.106Get rights and content

Abstract

According to the principle of excited fluorescence of organic compounds, the fluorescence properties of boscalid were studied. The mixed system of grape juice and boscalid was studied by fluorescence spectrometer. The fluorescence spectra of different pesticides contents in grape juice were obtained and the relationship between fluorescence intensity and pesticide contentwas analyzed. The results showed that when 292nm was used as the excitation wavelength, there was almost no fluorescence peak in grape juice, and the strong fluorescence characteristic peak of grape juice with boscalid was at 356nm. The prediction model of the content of boscalid in grape juice was obtained by dilution of boscalid in grape juice. The correlation coefficient was 0.9999, the recovery was 98 ∼ 105%, the average recovery was 101.7%, and the relative standard deviation was 2.5723%.

Introduction

Boscalid is a new type of nicotinamide fungicides, which is very effective in controlling powdery mildew, gray mold, sclerotia and various kinds of rot diseases. Boscalid is mainly used to control diseases including fruit trees, vegetables, rapeseed, grapes and field crops. The large amount of boscalid is used in agricultural production, which will lead to large pesticide residues in soil, plants and water bodies, which will directly harm the environment and human health. Most people focus on the environment or agricultural products, but ignore the pesticide residue detection of processed agricultural products, such as jam, juice drinks and so on. More attention should be paid to pesticide residues in these products.

At present, there are many methods for the detection of boscalid pesticide residues [1,2], including thin layer chromatography, high performance liquid chromatography, gas chromatography [3,4] and biochemical method [5,6]. These methods have many shortcomings, such as consuming more reagents, and the treatment process is cumbersome, so it is not possible to realize nondestructive testing of substances. Fluorescence analysis [[7], [8], [9], [10], [11], [12], [13]] has the advantages of simple operation, high sensitivity, good selectivity, fast analysis and nondestructive testing. In this paper, LS55 fluorescence spectrophotometer was used to detect and study the pesticide residues of boscalid in grape juice, and the fluorescence characteristics of boscalid in grape juice were analyzed.

Section snippets

Instruments

LS55 fluorescence spectrophotometer, using ozone free pulsed xenon lamp, excitation light range 200–800 nm, emission light range 200–900 nm, standard photomultiplier tube measurable range 200–650 nm, optional R928 red-sensitive photomultiplier tube extended to 900 nm. The instrument used 1 cm quartz cuvette. Slit variable: excitation path 2.5–15 nm, emission path2.5–20 nm, step distance 0.1 nm, can be measured fluorescence, phosphorescence, bioluminescence or chemiluminescence.

Materials

100% grape juice,

Experimental method for fluorescence spectrometric detection

Configure the sample solution, extract it and put it in an aquartz cuvette. LS55 fluorescence spectrophotometer was used to detect the sample. Then determine the excitation wavelength of the sample. The width of the slit is 5.0 nm, the scanning speed is 500 nm/min, the scanning wavelength is 200∼600 nm.

Fluorescence spectrum detection

The boscalid pesticide was dissolved in the diluted grape juice and the concentration was diluted to 0.02721 mg/ml, 0.01419 mg/ml with purified water. 292 nm was selected as the excitation

Conclusion

According to the analysis results, the quantitative and qualitative detection of the concentration of boscalid in grape juice can be realized by using fluorescence spectrum. The results showed that there was a very obvious characteristic fluorescence peak of boscalid in contrast to grape juice when excited by 292 nm. Therefore, fluorescence characteristic peaks can be used to detect and analyze the content of boscalid in grape juice. The fluorescence spectra of the mixed system of grape juice

Acknowledgements

Funding: This work was supported by the National Natural Science Foundation Project of China (61704063 and 61801188); Jiangsu provincial university natural science research major program (17KJA510001); The school-level achievement conversion special fund and natural science research program of Huaiyin Institute of Technology (16HGZ004); Jiangsu Overseas Visiting Scholar Program for University Prominent Yong; Jiangsu University “Blue Project” Funding.

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