Letter to the EditorKCa1.1 β4-subunits are not responsible for iberiotoxin-resistance in baroreceptor neurons in adult male rats
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Conflict of interests
The authors have declared that no competing interest exists.
Acknowledgment
This project was supported by research grants from the National Natural Science Foundation of China (81173051; 31171122; 31400983).
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Estrogen-dependent MicroRNA-504 Expression and Related Baroreflex Afferent Neuroexcitation via Negative Regulation on KCNMB4 and KCa1.1 β4-subunit Expression
2020, NeuroscienceCitation Excerpt :Finally, there is almost no distribution of myelinated Ah-types in adult males except for A- and C-types (Li and Schild, 2007; Li et al., 2008). Therefore, single-cell qRT-PCR tests (Li et al., 2015; Xu et al., 2015; Liu et al., 2016) were carried out using electrophysiologically and morphometrically identified individual BRNs (Li and Schild, 2007; Lu et al., 2013) (Fig. 6A) isolated from adult female rats underwent aortic depressor nerve (ADN) fluorescent dye (Dil) labeling to identify the baroreceptor phenotype (Lu et al., 2013). These data indicated that myelinated A-, Ah- and unmyelinated C-type BRNs expressed both α- (n = 9/17, 8/10, and 11/13, respectively, for each category) and β4-subunits (n = 7/13, 8/9, and 10/11, respectively) (Fig. S8) and the percentage of positive qRT-PCR detection was 52.9%, 80.0%, and 84.6% for α-subunits and 53.8%, 88.9%, and 83.3% for β4-subunits, respectively, suggesting that less population of A-types expresses both subunits (Fig. 6B-C).
FGF-21 ameliorates essential hypertension of SHR via baroreflex afferent function
2020, Brain Research BulletinKCa1.1 is potential marker for distinguishing Ah-type baroreceptor neurons in NTS and contributes to sex-specific presynaptic neurotransmission in baroreflex afferent pathway
2015, Neuroscience LettersCitation Excerpt :Following the classification, the stimulation from a concentric bipolar electrode (Fig. 1B, indicated by▾) was applied and solitary track-evoked EPSCs were also recorded in Cap-insensitive (presumable A-type) NTS neurons before and after 100 nM iberiotoxin (IbTX), a selective KCa1.1 channel blocker, on top of CTZ. The results (Fig. 1B) showed that the peak, DTC, and AUC were dramatically increased in the presence of CTZ but was not further enhanced by IbTX (Fig. 1B–E), suggesting, in accordance with our previous data collected from the 1st-order neurons [2,8], that the myelinated/Cap-insensitive afferent does not contribute to the enhanced neurotransmission by increasing glutamate release via blocking KCa1.1 channel. In the case of Cap-sensitive NTS (presumable C-type) neurons, the hyperpolarization induced a significant delay excitation (Fig. 2A, indicated by ) and the peak, DTC, and AUC were all further increased (P < 0.05 or P < 0.01 vs CTZ alone) in the presence of 100 nM IbTX after initial increases by removing the rapid desensitization with 100 μM CTZ (Fig. 2B–E), These results suggest that blocking KCa1.1 play a role in unmyelinated neurotransmission and markedly enhance the excitatory neurotransmission by upregulation of pre-synaptic release of glutamate.
Perfect cellular platform for electrophysiology: Acutely isolated vagal ganglion neurons from adult canines
2020, Chinese Journal of Pharmacology and Toxicology
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These authors contributed equally to this work.