Full length articleEffects of dietary Spirulina platensis on growth performance, hematological and serum biochemical parameters, hepatic antioxidant status, immune responses and disease resistance of Coral trout Plectropomus leopardus (Lacepede, 1802)
Introduction
Coral trout Plectropomus leopardus is an important economical marine fish species in China [1]. Recent years, due to its successful development of technique for fry artificial breeding, P. leopardus aquaculture has developed rapidly and widely along the southern coast of China in tropical and subtropical climates [2]. However, when farmed P. leopardus suffers from stress conditions such as high rearing density and poor water quality, it is more susceptible to several pathogens than its wild counterpart. Diseases occurred more frequently resulting in serious economic losses to Province Hainan, especially between July and September every year [3,4]. Therefore, antibiotics, vaccines and some immunostimulants have been used to prevent disease outbreaks in P. leopardus aquaculture [5]. However, the intensive use of antibiotics to control the bacterial diseases in aquaculture has led to an increase in antibiotic resistance which is a threat to human health, and the application of vaccines is quite expensive [6,7]. Additionally, antibiotics can kill beneficial intestinal microbial flora and interfere the innate ecosystem which affects the physiology and immunity of fish [8,9]. To avoid such adverse situation, ecofriendly immunostimulants such as microalgae and probiotics have been studied to improve the growth performance, immune responses and disease resistance of aquatic animals [[10], [11], [12]].
Spirulina platensis is a helicoidal, unbranched and filamentous cyanobacterium that composes of various nutritional components, such as antioxidant pigments, essential amino acids, essential fatty acids, vitamins and minerals [13,14]. In previous studies, many researchers have confirmed that supplementation of S. platensis has positive effects on growth performance, skin pigmentation and body composition in different fish species, such as Guppy Poecilia reticulata [15], African Sharptooth Catfish Clarias gariepinus [16], Golden Pompano Trachinotus ovatus [17] and Queen loach Botia dario [18]. In recent years, S. platensis received more attention mainly as it can improve immunity and disease resistance of Nile Tilapia Oreochromis niloticus [19,20], Great Sturgeon Huso huso [21] and Olive Flounder Paralichthys olivaceus [22].
However, little is known about the effects of S. platensis on growth performance, antioxidant abilities, immunity and disease resistance for P. leopardus. Therefore, the purpose of the present study was to evaluate the effects of dietary S. platensis on growth performance, hematological and serum biochemical parameters, hepatic antioxidant status, immune responses and disease resistance to the pathogen infection of P. leopardus, which would encourage its use as a potential immunostimulant to feed additive.
Section snippets
Experimental diets
Since the crude protein level of S. platensis and soy protein concentrate was nearly similar [17], the dietary soy protein concentrate was substituted for the alga. Six diets were formulated to contain 0% (C), 2% (SP2), 4% (SP4), 6% (SP6), 8% (SP8) and 10% (SP10) S. platensis (Yuequn Ocean Biological Research Development Co., Guangdong, China). Ingredients and proximate compositions of the experimental diets were presented in Table 1. Ingredients were weighed according to the feed formulation,
Effects of dietary S. platensis on growth performance in P. leopardus
Growth performance of fish fed with different experimental diets was shown in Table 2. Both WGR and SGR were apeared a dose-dependent increase, and the highest WGR and SGR were observed in 10% S. platensis enriched diet (P < .05). On the other hand, dose related decreasing was observed in FCR, and the highest decrease was detected in SP10 group. The CF ranged from 2.42 to 2.65, also the SR fluctuated from 95.65% to 97.41%. No significant variations were detected on the CF and SR among all
Discussion
During the feeding trial, all the experimental diets containing the S. platensis were well accepted by the experimental fish. The growth of fish showed increasing trends with 2–10% of the S. platensis supplemented, and significant differences were observed in WGR and SGR among SP10, SP8 and C groups (P < .05). In the present study, we observed a significant increase of WGR and SGR in fish fed diets supplemented with 10% S. platensis for 8 weeks. The result revealed that the fish improved growth
Conclusion
In conclusion, our study indicated that P. leopardus fed a diet supplemented with S. platensis (especially at 10% of fed supplement) for 8 weeks improved its growth performance; promoted its hepatic antioxidant status by increasing hepatic T-AOC; enhanced immune ability by increasing LYZ activity, RBA, Ig content, complement content and the levels of hematological parameters (RBC, WBC and Hb); decreased the levels of cholesterol and triglyceride. Additionally, dietary supplementation of S.
Acknowledgements
This project was financially supported by the Central Public-interest Scientific Institution Basal Research Fund, South China Sea Fisheries Research Institute, CAFS (NO. 2018 ZD 01, NO. 2015 TS 19 and NO. 2015 TS 20), the Special Scientific Research Funds for public welfare industry (Agriculture NO. 201403011).
References (69)
- et al.
The Effects of dietary soybean isoflavones on growth, innate immune responses, hepatic antioxidant abilities and disease resistance of juvenile golden pompano Trachinotus ovatus
Fish Shellfish Immunol.
(2015) - et al.
Effect of germ-free rearing environment on gut development of larval sea bass (Dicentrarchus labrax L.)
Aquaculture
(2009) - et al.
Recent advances in the role of probiotics and prebiotics in carp aquaculture: a review
Aquaculture
(2016) - et al.
Comparison effect of dietary astaxanthin and Haematococcus pluvialis on growth performance, antioxidant status and immune response of large yellow croaker Pseudosciaena crocea
Aquaculture
(2014) Catalase in vitro
Methods Enzymol.
(1984)- et al.
Microsomal lipid peroxidation
Methods Enzymol.
(1978) - et al.
Changes in plasma lysozyme and blood leucocyte levels of hatchery-reared Atlantic salmon (Salmo salar L.) and sea trout (Salmo trutta L.) during parr-smolt transformation
Aquaculture
(1992) - et al.
Effect of hyperoxygenation and low water flow on the primary stress response and susceptibility of Atlantic salmon Salmo salar L. to experimental challenge with IPN virus
Aquaculture
(2007) - et al.
Non-invasive measurement of cortisol and melatonin in tanks stocked with seawater Atlantic salmon
Aquaculture
(2007) - et al.
Selenite causes cytotoxicity in rainbow trout (Oncorhynchus mykiss) hepatocytes by inducing oxidative stress
Toxicol In Vitro
(2009)
Blood antioxidant defenses and hematological adjustments in crowded/uncrowded rainbowtrout (Oncorhynchus mykiss) fed on diets with different levels of antioxidant vitamins and HUFA
Aquaculture
The effect of dietary supplementation with blueberry, a-tocopherol or astaxanthin on oxidative stability of Arctic char (Salvelinus alpinus) semen
Theriogenology
Effects of anthraquinone extract from Rheum officinale Bail on the growth performance and physiological responses of Macrobrachium rosenbergii under high temperature stress
Fish Shellfish Immunol.
Effect of dietary isoleucine on the immunity, antioxidant status, tight junctions and microflora in the intestine of juvenile Jian carp (Cyprinus carpio var. Jian)
Fish Shellfish Immunol.
Noncellular nonspecific defense mechanisms of fish
Annu. Rev. Fish Dis.
Immune responses and stress resistance in red sea bream, Pagrus major, after oral administration of heat-killed Lactobacillus plantarum and vitamin C
Fish Shellfish Immunol.
Effects of dietary supplementation of spirulina and quercetin on growth, innate immune responses, disease resistance against edwardsiella tarda, and dietary antioxidant capacity in the Juvenile Olive Flounder Paralichthys olivaceus
Fish Aquat. Sci.
Dietary lipid requirement on non-specific immune responses in juvenile grass carp (Ctenopharyngodon idella)
Fish Shellfish Immunol.
Replacement of frozen fish meat based diet with artificial diets in rearing of coral trout Plectropomus leopardus (Lacepede, 1802) fingerlings
Indian J. Fish.
Effects of light color on growth, skin color, and physiological indices of juvenile Plectropomus leopardus in a recirculating aquaculture system
J. Fish. Sci. China
Isolation of pathogenic listonella anguillarum from Plectropomus leopardus and its biological characterization
P. Ocean Univ. China
Identification and drug sensitive test of bacterial Pathogens from Plectropomus leopardus with tail fester disease
South China Fish. Sci.
Transfer of multidrug-resistant bacteria between intermingled ecological niches: the interface between humans, animals and the environment
Int. J. Environ. Res. Publ. Health
Multidrug resistance: an emerging crisis
Int. Discip Perspect Infect Dis
Reciprocal interactions of the intestinal microbiota and immune system
Nature
Enzyme-producing bacteria isolated from fish gut: a review
Aquacult. Nutr.
Effects of dietary peppermint (Mentha piperita) on growth performance, chemical body composition and hematological and immune parameters of fry Caspian white fish (Rutilus frisii kutum)
Fish Shellfish Immunol.
Algae
Spirulina as a natural carotenoid source on growth, pigmentation and reproductive performance of yellow tail cichlid Pseudotropheus acei
Aquacult. Int.
Effect of dietary supplementation of different rates of spirulina (Spirulina platensis) on growth and fed conversion in Guppy(Poecilia reticulata)
J. Anim. Vet. Adv.
The effects of spirulina platensis and Cladophora algae on the growth performance, meat quality and immunity stimulating capacity of the african Sharptooth catfish (Clarias gariepinus)
Int. J. Agric. Biol.
Effect of different levels of Spirulina platensis dietary supplementation on the growth, body color, digestion, and immunity of Trachinotus ovatus,9 pages
Isr. J. Aquacult.- Bamidgeh, IJA
Effect of dietary Wolffia arrhiza and Spirulina platensis on growth performance and pigmentation of Queen loach Botia dario (Hamilton, 1822)
Aquacult. Nutr.
The role of Spirulina platensis (Arthrospira platensis) in growth and immunity of Nile Tilapia (Oreochromis niloticus) and its resistance to bacterial infection
J. Agric. Sci.
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