Original articleA comparison of the effects of N-acetylcysteine and ethyl pyruvate on experimental testicular ischemia-reperfusion injury
Section snippets
Study Design
This was a randomized, controlled, nonblinded interventional animal study, the protocol for which was approved by the Karadeniz Technical University Animal Care and Ethics Committee.
Setting and Selection of Subjects
Twenty-four mature male Wistar rats (10 weeks old, weighing 240–280 g) were used. The animals were kept in steel cages until the day of the study, at a room temperature of 22°C and were given water and standard rat chow. Only water was provided for the last 12 hours before the study.
Intervention
The 24 rats were randomized into
Results
Twenty-four rats were included in the study. One rat from the control group died during the monitoring period.
According to our results, the median IMA levels were measured as 0.793, 0.864, 0.874, and 0.775 absorbance units and the median histopathological score were obtained as 9.0, 7.3, 9.3, and 9.4 point in the control, T/D, NAC, and EP groups, respectively. Levels of serum IMA, MDA, MPO, and tissue testicular MPO and MDA levels are summarized in Table 1.
On the basis of our results, distinct
Discussion
The major purposes of our study were to investigate whether NAC and EP reverse I/R injuries after testicular T/D and to compare them using histopathological and new biochemical techniques. According to our results, significant I/R-associated histopathological injury developed when T/D was administered, and EP and NAC exhibited a protective effect against that injury. Because this suggested that increased IMA levels might be the biochemical parameter that best reflects I/R injury in the testes,
Acknowledgment
The authors thank Omer Kutlu, M.D., Ahmet Alver, Ph.D., and Abdulkadir Gunduz, M.D., for their comments.
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2020, Journal of Pediatric SurgeryCitation Excerpt :This difference could be because of sampling time (They sampled at 2 h; we sampled at 30 days). Turkmen et al. noted that the Johnsen score at shorter sampling time (2 h) reflects only the current condition of germ cells [3]. We also believe the Johnsen score at a shorter sampling time (2 h) would not show germ cell production capacity of the testis.
Berberine inhibits the ischemia-reperfusion induced testicular injury through decreasing oxidative stress
2020, American Journal of Emergency MedicineCitation Excerpt :Approximately 25% of men with a history of torsion will experience adult-onset infertility [1]. Although testicular recovery with surgical detorsion rates have been reported to be between 42 and 88%, it is unclear whether the functions are fully conserved [9]. Therefore, it is clinically important to find therapeutic applications as additional treatment in surgical repair [1].
N-acetylcysteine protects testicular tissue against ischemia/reperfusion injury via inhibiting endoplasmic reticulum stress and apoptosis
2019, Journal of Pediatric UrologyCitation Excerpt :In recent years, several pharmacological agents, such as phosphodiesterase inhibitors, vitamin C and E, selenium, flavonoids, NSAIDs, ethyl pyruvate, and NAC, have been investigated for their potential as an adjunctive therapy in the surgical repair of TT in animal models. These agents usually contain anti-inflammatory, antioxidant, or ROS scavenging properties [2,8,16]. N-acetylcysteine has been widely used in many studies related to I/R injury because of its antioxidant properties [8,16].
S.T. has nothing to disclose. A.M. has nothing to disclose. E.K. has nothing to disclose. Y.K. has nothing to disclose. A.K. has nothing to disclose. A.U. has nothing to disclose. E.Y. has nothing to disclose. S.T. has nothing to disclose.