Cell Metabolism
Volume 30, Issue 5, 5 November 2019, Pages 963-975.e7
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Short Article
Proteomics-Based Comparative Mapping of the Secretomes of Human Brown and White Adipocytes Reveals EPDR1 as a Novel Batokine

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Highlights

  • Identification of the first human brown fat secretome

  • Comparative analysis of the secretomes of human brown and white adipocytes

  • 101 proteins were exclusively identified in the secretome of brown adipocytes

  • EPDR1 is a novel batokine important for brown fat commitment

Summary

Adipokines secreted from white adipose tissue play a role in metabolic crosstalk and homeostasis, whereas the brown adipose secretome is less explored. We performed high-sensitivity mass-spectrometry-based proteomics on the cell media of human adipocytes derived from the supraclavicular brown adipose and from the subcutaneous white adipose depots of adult humans. We identified 471 potentially secreted proteins covering interesting categories such as hormones, growth factors, extracellular matrix proteins, and proteins of the complement system, which were differentially regulated between brown and white adipocytes. A total of 101 proteins were exclusively quantified in brown adipocytes, and among these was ependymin-related protein 1 (EPDR1). EPDR1 was detected in human plasma, and functional studies suggested a role for EPDR1 in thermogenic determination during adipogenesis. In conclusion, we report substantial differences between the secretomes of brown and white human adipocytes and identify novel candidate batokines that can be important regulators of human metabolism.

Keywords

human batokines
human brown fat
human brown adipocytes
secretomics
extracellular matrix proteins
adipokines
brown fat differentiation
brown fat commitment
mitochondria
adipocyte complement factors

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These authors contributed equally

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